Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1686.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 314.79 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 140.38
A375-P4
B11
COLO 858 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 442.01 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 173.57 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 141.54
Colo858-P3
D11
A-375 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2722.08 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 510.86 NGFR rabbit IgG(H+L) / donkey NGFR 141.68
A375-P1
B10
WM-115 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 877.78 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 192.37 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 141.74
WM115-P4
E2
COLO 858 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2017.22 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 307.47 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 141.78
Colo858-P4
B9
COLO 858 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3260.79 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 400.81 NGFR rabbit IgG(H+L) / donkey NGFR 142.45
Colo858-P7
B2
COLO 858 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2512.53 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 349.85 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 142.66
Colo858-P9
B2
WM-115 Vemurafenib Palbociclib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 73.48 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 51.63 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 142.88
WM115-P10
D11
A-375 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2061.35 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 372.92 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 142.91
A375-P10
E8
A-375 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1529.11 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 298.67 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 142.92
A375-P4
E11
A-375 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2008.37 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 365.17 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 143.22
A375-P10
B8
COLO 858 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2708.17 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 393.16 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 143.44
Colo858-P9
B3
A-375 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 580.03 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 130.73 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 143.65
A375-P9
B11
COLO 858 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2555.33 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 349.95 NGFR rabbit IgG(H+L) / donkey NGFR 143.72
Colo858-P1
E3
A-375 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2096.28 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 413.41 NGFR rabbit IgG(H+L) / donkey NGFR 143.89
A375-P7
B9
COLO 858 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2259.14 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 363.05 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 145.26
Colo858-P10
B2
WM-115 Vemurafenib Saracatinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 442.79 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 92.31 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 146.15
WM115-P10
G3
WM-115 Vemurafenib Pictilisib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 499.81 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 115.87 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 146.91
WM115-P9
B5
COLO 858 Vemurafenib Saracatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1344.98 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 220.89 NGFR rabbit IgG(H+L) / donkey NGFR 147.29
Colo858-P7
C3
WM-115 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 861.21 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 191.82 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 148.17
WM115-P4
E3
COLO 858 Vemurafenib Defactinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 493.23 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 127.77 NGFR rabbit IgG(H+L) / donkey NGFR 149.72
Colo858-P1
G7
WM-115 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 312.72 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 97.94 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 150.06
WM115-P4
D4
WM-115 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 344.87 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 74.07 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 151.25
WM115-P10
G2
WM-115 Vemurafenib Pictilisib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 675.49 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 168.69 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 151.72
WM115-P10
B4
COLO 858 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2543.56 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 325.24 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 151.83
Colo858-P3
E3