Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2657.88 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 504.90 NGFR rabbit IgG(H+L) / donkey NGFR 261.89
A375-P7
E2
COLO 858 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3684.23 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 505.69 NGFR rabbit IgG(H+L) / donkey NGFR 115.96
Colo858-P1
B10
A-375 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2322.28 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 508.09 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 7094.33
A375-P8
E2
A-375 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2722.08 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 510.86 NGFR rabbit IgG(H+L) / donkey NGFR 141.68
A375-P1
B10
A-375 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2440.77 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 511.76 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 8412.88
A375-P2
B10
A-375 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2981.15 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 513.45 NGFR rabbit IgG(H+L) / donkey NGFR 573.44
A375-P1
B3
A-375 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2998.94 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 515.01 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 5982.45
A375-P2
E3
A-375 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3009.25 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 525.64 NGFR rabbit IgG(H+L) / donkey NGFR 196.08
A375-P7
E3
A-375 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2988.24 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 526.60 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 6337.37
A375-P2
E2
A-375 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3036.79 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 535.40 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 6341.83
A375-P2
B2
A-375 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2781.07 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 536.04 NGFR rabbit IgG(H+L) / donkey NGFR 281.52
A375-P7
B2
A-375 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3098.11 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 540.77 NGFR rabbit IgG(H+L) / donkey NGFR 567.23
A375-P1
E2
A-375 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3094.28 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 552.89 NGFR rabbit IgG(H+L) / donkey NGFR 593.97
A375-P1
B2
A-375 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3238.32 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 574.69 NGFR rabbit IgG(H+L) / donkey NGFR 547.66
A375-P1
E3
A-375 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3073.51 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 581.89 NGFR rabbit IgG(H+L) / donkey NGFR 205.60
A375-P7
B3