Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2597.12 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 404.96 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 265.99
A375-P4
B2
COLO 858 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3383.30 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 405.53 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 96.01
Colo858-P3
B11
A-375 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2231.52 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 405.65 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 204.22
A375-P10
E3
A-375 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2223.28 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 406.34 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 214.00
A375-P10
E2
A-375 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2181.70 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 406.40 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 169.81
A375-P4
B10
A-375 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2413.32 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 408.16 NGFR rabbit IgG(H+L) / donkey NGFR 329.47
A375-P7
E8
COLO 858 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2571.01 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 408.55 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 5297.17
Colo858-P8
B9
COLO 858 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2604.12 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 409.44 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 5110.94
Colo858-P8
E3
A-375 Vemurafenib Pictilisib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2053.10 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 410.35 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 4952.05
A375-P8
E4
COLO 858 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3266.62 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 411.78 NGFR rabbit IgG(H+L) / donkey NGFR 137.05
Colo858-P7
B9
A-375 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2565.82 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 412.30 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 260.81
A375-P4
E3
COLO 858 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3490.89 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 412.36 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 99.67
Colo858-P4
B11
A-375 Vemurafenib Pictilisib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2012.07 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 413.37 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 5072.92
A375-P8
B4
A-375 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2096.28 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 413.41 NGFR rabbit IgG(H+L) / donkey NGFR 143.89
A375-P7
B9
A-375 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1854.98 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 413.60 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 7390.58
A375-P2
B11
COLO 858 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3387.57 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 414.22 NGFR rabbit IgG(H+L) / donkey NGFR 87.43
Colo858-P1
B11
A-375 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2682.85 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 414.64 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 436.39
A375-P3
B2
A-375 Vemurafenib Defactinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2291.41 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 418.38 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 377.28
A375-P3
B6
A-375 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2063.42 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 420.07 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 6743.66
A375-P8
E9
A-375 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2483.07 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 422.64 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 223.37
A375-P3
E10
A-375 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2194.35 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 424.25 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 5871.83
A375-P8
E8
COLO 858 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3607.06 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 427.25 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 98.07
Colo858-P4
E11
A-375 Vemurafenib Pictilisib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2339.94 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 429.64 NGFR rabbit IgG(H+L) / donkey NGFR 360.80
A375-P7
E4
A-375 Vemurafenib Defactinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2351.55 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 431.86 NGFR rabbit IgG(H+L) / donkey NGFR 295.59
A375-P1
E6
A-375 Vemurafenib PF562271 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2421.26 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 433.32 NGFR rabbit IgG(H+L) / donkey NGFR 252.56
A375-P1
E8