Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Pictilisib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1009.48 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 222.20 NGFR rabbit IgG(H+L) / donkey NGFR 765.25
Colo858-P7
D5
COLO 858 Vemurafenib Saracatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 622.82 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 222.22 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 521.65
Colo858-P9
F3
COLO 858 Vemurafenib Pictilisib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1375.08 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 222.63 NGFR rabbit IgG(H+L) / donkey NGFR 642.79
Colo858-P7
C4
COLO 858 Vemurafenib Pictilisib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 620.58 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 222.71 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 484.31
Colo858-P9
C4
COLO 858 Vemurafenib Pictilisib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 587.51 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 222.89 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 543.83
Colo858-P9
F4
WM-115 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 786.81 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 223.56 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 3331.33
WM115-P8
E8
COLO 858 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1526.64 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 224.83 NGFR rabbit IgG(H+L) / donkey NGFR 82.51
Colo858-P7
E11
COLO 858 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1053.34 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 226.13 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 692.16
Colo858-P3
F2
A-375 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 985.54 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 226.86 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 90.96
A375-P8
C6
A-375 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 892.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 227.01 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 314.05
A375-P10
C6
COLO 858 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 499.31 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 227.65 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 121.00
Colo858-P3
G11
A-375 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1015.43 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 227.72 NGFR rabbit IgG(H+L) / donkey NGFR 506.01
A375-P7
C6
COLO 858 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 654.62 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 227.86 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 681.06
Colo858-P9
D2
COLO 858 Vemurafenib JNK-IN-8 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1450.25 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 227.96 NGFR rabbit IgG(H+L) / donkey NGFR 177.02
Colo858-P7
C8
WM-115 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1053.21 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 228.32 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 3110.03
WM115-P2
E2
A-375 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1112.72 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 228.94 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 6005.37
A375-P8
E10
WM-115 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 849.20 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 229.62 NGFR rabbit IgG(H+L) / donkey NGFR 923.15
WM115-P7
E9
A-375 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1022.20 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 229.98 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 2502.46
A375-P2
F3
COLO 858 Vemurafenib AZD8055 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1564.84 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 230.30 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 166.92
Colo858-P9
E6
A-375 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 684.84 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 231.07 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 929.36
A375-P8
F9
COLO 858 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 828.41 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 231.09 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 648.00
Colo858-P9
C2
COLO 858 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1188.52 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 232.45 NGFR rabbit IgG(H+L) / donkey NGFR 555.83
Colo858-P1
F2
WM-115 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 989.36 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 234.08 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 3304.49
WM115-P2
E3
WM-115 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 885.39 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 234.32 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 206.30
WM115-P9
E8
A-375 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1042.51 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 234.63 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 2631.95
A375-P2
F2