Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Pictilisib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 703.08 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 176.42 NGFR rabbit IgG(H+L) / donkey NGFR 642.42
A375-P7
D4
WM-115 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 931.21 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 176.80 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 131.68
WM115-P10
B7
A-375 Vemurafenib Defactinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 262.84 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 177.01 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 340.33
A375-P4
D7
COLO 858 Vemurafenib JNK-IN-8 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 532.79 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 177.02 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 248.12
Colo858-P9
F8
WM-115 Vemurafenib PF562271 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 681.48 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 177.16 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 225.77
WM115-P3
B8
COLO 858 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 499.81 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 177.21 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 372.58
Colo858-P2
E4
WM-115 Vemurafenib Defactinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 625.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 177.31 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 254.54
WM115-P3
B7
COLO 858 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1561.00 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 177.47 NGFR rabbit IgG(H+L) / donkey NGFR 84.88
Colo858-P7
E7
COLO 858 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 509.46 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 177.66 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 607.02
Colo858-P9
G2
COLO 858 Vemurafenib Pictilisib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 619.69 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 177.76 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 210.99
Colo858-P8
F4
COLO 858 Vemurafenib Trametinib 1.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 509.49 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 177.92 NGFR rabbit IgG(H+L) / donkey NGFR 1008.58
Colo858-P1
D5
A-375 Vemurafenib AZD8055 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 534.00 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 177.96 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 299.97
A375-P10
D6
COLO 858 Vemurafenib PF562271 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 597.72 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 178.20 NGFR rabbit IgG(H+L) / donkey NGFR 290.02
Colo858-P1
C8
A-375 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 559.44 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 178.24 NGFR rabbit IgG(H+L) / donkey NGFR 158.24
A375-P1
C11
WM-115 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 996.48 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 178.28 NGFR rabbit IgG(H+L) / donkey NGFR 1205.16
WM115-P7
E7
COLO 858 Vemurafenib Pictilisib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 367.86 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 178.35 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 482.06
Colo858-P9
G5
WM-115 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 639.56 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 178.56 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 176.26
WM115-P3
E11
A-375 Vemurafenib Defactinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 591.99 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 179.12 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1592.32
A375-P2
C6
A-375 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 673.31 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 179.29 NGFR rabbit IgG(H+L) / donkey NGFR 398.37
A375-P7
B11
COLO 858 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 702.53 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 179.40 NGFR rabbit IgG(H+L) / donkey NGFR 1135.60
Colo858-P1
F4
WM-115 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 824.81 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 179.64 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 159.69
WM115-P9
B2
COLO 858 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 640.45 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 179.90 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 721.38
Colo858-P2
D11
COLO 858 Vemurafenib 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 818.13 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 180.10 NGFR rabbit IgG(H+L) / donkey NGFR 774.72
Colo858-P1
G2
COLO 858 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 785.10 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 180.24 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 189.75
Colo858-P9
E11
A-375 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 790.57 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 180.60 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 527.06
A375-P9
C6