Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 515.65 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 159.53 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 579.91
A375-P2
F9
COLO 858 Vemurafenib AZD8055 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 242.10 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 159.62 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 57.07
Colo858-P8
D6
A-375 Vemurafenib Dasatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 468.48 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 159.69 NGFR rabbit IgG(H+L) / donkey NGFR 243.06
A375-P1
G10
WM-115 Vemurafenib PF562271 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 521.81 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 159.77 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 121.47
WM115-P4
B8
A-375 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 472.08 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 159.91 NGFR rabbit IgG(H+L) / donkey NGFR 749.94
A375-P7
C10
A-375 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 500.28 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 160.02 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 381.20
A375-P8
D9
COLO 858 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 830.58 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 160.02 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 78.71
Colo858-P8
E7
A-375 Vemurafenib Pictilisib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 589.31 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 160.08 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 343.41
A375-P9
G5
WM-115 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 588.33 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 160.22 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 96.17
WM115-P10
B3
COLO 858 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 402.10 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 160.23 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 920.59
Colo858-P3
B4
A-375 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 448.26 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 160.44 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 208.39
A375-P4
G11
A-375 Vemurafenib JNK-IN-8 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 413.18 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 160.47 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 115.79
A375-P10
C8
A-375 Vemurafenib 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 630.43 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 160.53 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1364.66
A375-P2
G2
COLO 858 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 999.15 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 160.59 NGFR rabbit IgG(H+L) / donkey NGFR 195.26
Colo858-P7
D8
A-375 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 466.25 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 160.61 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1844.28
A375-P2
B4
COLO 858 Vemurafenib Defactinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 590.59 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 160.66 NGFR rabbit IgG(H+L) / donkey NGFR 90.87
Colo858-P1
F7
COLO 858 Vemurafenib PF562271 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 460.90 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 160.93 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 514.91
Colo858-P3
D9
A-375 Vemurafenib Pictilisib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 566.92 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 161.07 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 138.17
A375-P8
G5
A-375 Vemurafenib Defactinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 576.51 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 161.13 NGFR rabbit IgG(H+L) / donkey NGFR 458.75
A375-P1
G6
COLO 858 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 314.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 161.24 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 74.70
Colo858-P10
G9
COLO 858 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 351.99 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 161.36 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 575.58
Colo858-P3
C9
A-375 Vemurafenib Pictilisib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 541.14 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 161.66 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 366.57
A375-P9
D5
A-375 Vemurafenib Saracatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 707.83 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 161.86 NGFR rabbit IgG(H+L) / donkey NGFR 226.02
A375-P7
F3
WM-115 Vemurafenib PF562271 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 644.33 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 162.03 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 254.66
WM115-P3
E8
A-375 Vemurafenib Pictilisib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 533.47 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 162.30 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 124.78
A375-P8
D5