Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 573.25 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 137.42 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 113.14
A375-P10
B11
WM-115 Vemurafenib Defactinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 455.93 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 137.48 NGFR rabbit IgG(H+L) / donkey NGFR 1254.85
WM115-P1
C7
WM-115 Vemurafenib 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 577.06 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 137.57 NGFR rabbit IgG(H+L) / donkey NGFR 2587.63
WM115-P1
D3
A-375 Vemurafenib PF562271 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 318.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 137.65 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 268.61
A375-P3
D9
WM-115 Vemurafenib Defactinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 387.18 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 137.65 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 523.09
WM115-P3
C6
COLO 858 Vemurafenib Palbociclib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 820.67 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 137.82 NGFR rabbit IgG(H+L) / donkey NGFR 1062.99
Colo858-P7
G10
COLO 858 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 430.32 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 137.82 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 435.39
Colo858-P4
D4
A-375 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 318.19 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 137.96 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 175.13
A375-P4
C11
WM-115 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 498.94 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 138.13 NGFR rabbit IgG(H+L) / donkey NGFR 2782.79
WM115-P1
B4
COLO 858 Vemurafenib Trametinib 0.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 390.78 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 138.15 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 318.20
Colo858-P2
E5
COLO 858 Vemurafenib AZD8055 1.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 345.85 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 138.16 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 741.60
Colo858-P9
G7
A-375 Vemurafenib Defactinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 436.80 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 138.25 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 894.41
A375-P2
F7
COLO 858 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 326.69 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 138.54 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 734.57
Colo858-P2
C9
A-375 Vemurafenib Defactinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 492.21 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 138.59 NGFR rabbit IgG(H+L) / donkey NGFR 204.04
A375-P1
F7
A-375 Vemurafenib Palbociclib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 380.71 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 138.61 NGFR rabbit IgG(H+L) / donkey NGFR 692.41
A375-P7
G10
WM-115 Vemurafenib Dasatinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 476.13 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 138.74 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 340.95
WM115-P3
G11
A-375 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 536.34 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 138.84 NGFR rabbit IgG(H+L) / donkey NGFR 269.68
A375-P7
G2
WM-115 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 482.95 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 139.16 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 599.32
WM115-P2
F10
A-375 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 295.24 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 139.24 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 278.03
A375-P3
C9
A-375 Vemurafenib Defactinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 507.77 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 139.27 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 760.48
A375-P2
G6
A-375 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 369.03 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 139.31 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 75.47
A375-P10
D9
A-375 Vemurafenib JNK-IN-8 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 413.43 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 139.34 NGFR rabbit IgG(H+L) / donkey NGFR 264.58
A375-P7
C8
COLO 858 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 838.35 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 139.35 NGFR rabbit IgG(H+L) / donkey NGFR 802.84
Colo858-P7
C10
A-375 Vemurafenib Saracatinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 473.40 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 139.53 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 307.92
A375-P9
G3
COLO 858 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 401.40 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 139.60 NGFR rabbit IgG(H+L) / donkey NGFR 422.47
Colo858-P1
D8