Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 75.79 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 117.87 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 622.25
Colo858-P9
F10
A-375 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 236.14 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 117.87 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 347.87
A375-P4
D4
WM-115 Vemurafenib AZD8055 1.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 787.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 118.07 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 448.28
WM115-P9
G7
A-375 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 295.05 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 118.16 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 243.87
A375-P10
F10
WM-115 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 319.14 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 118.20 NGFR rabbit IgG(H+L) / donkey NGFR 1378.93
WM115-P7
B10
WM-115 Vemurafenib Defactinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 418.11 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 118.21 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 444.82
WM115-P3
F7
WM-115 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 453.28 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 118.64 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 249.06
WM115-P4
C3
A-375 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 283.15 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 118.66 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 82.01
A375-P10
G8
WM-115 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 453.85 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 118.93 NGFR rabbit IgG(H+L) / donkey NGFR 1722.13
WM115-P7
D9
COLO 858 Vemurafenib Defactinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 284.02 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 118.94 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 204.79
Colo858-P4
C7
COLO 858 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 372.17 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 119.07 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 261.05
Colo858-P4
G8
WM-115 Vemurafenib Trametinib 0.316 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 446.09 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 119.13 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 484.62
WM115-P3
F5
A-375 Vemurafenib PF562271 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 230.55 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 119.38 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 292.63
A375-P4
C8
WM-115 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 575.52 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 119.57 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 507.50
WM115-P3
F2
COLO 858 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 104.50 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 119.60 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 587.36
Colo858-P8
F11
A-375 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 457.50 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 119.63 NGFR rabbit IgG(H+L) / donkey NGFR 310.49
A375-P7
G8
WM-115 Vemurafenib Pictilisib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 489.76 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 119.68 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 183.88
WM115-P9
E4
WM-115 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 447.94 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 119.74 NGFR rabbit IgG(H+L) / donkey NGFR 2263.76
WM115-P1
G8
A-375 Vemurafenib Trametinib 0.316 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 182.46 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 119.76 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 395.54
A375-P4
C5
COLO 858 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 343.47 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 120.07 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 432.43
Colo858-P2
G8
WM-115 Vemurafenib Pictilisib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 519.33 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 120.21 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 214.27
WM115-P10
D4
A-375 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 411.56 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 120.50 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 209.21
A375-P9
F9
COLO 858 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 488.28 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 120.56 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 377.84
Colo858-P4
G4
A-375 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 266.86 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 120.57 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 332.97
A375-P4
F4
A-375 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 345.98 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 120.79 NGFR rabbit IgG(H+L) / donkey NGFR 369.72
A375-P7
D8