Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib Saracatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 401.73 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 111.52 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 171.77
WM115-P10
C3
WM-115 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 420.89 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 111.56 NGFR rabbit IgG(H+L) / donkey NGFR 1308.11
WM115-P7
C9
WM-115 Vemurafenib Saracatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 391.99 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 111.65 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 328.46
WM115-P9
C3
WM-115 Vemurafenib Saracatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 424.05 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 111.89 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1016.90
WM115-P8
C3
COLO 858 Vemurafenib Defactinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 246.23 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 112.14 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 209.45
Colo858-P4
D7
COLO 858 Vemurafenib Defactinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 303.50 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 112.14 NGFR rabbit IgG(H+L) / donkey NGFR 133.45
Colo858-P1
D7
A-375 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 290.36 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 112.32 NGFR rabbit IgG(H+L) / donkey NGFR 272.43
A375-P7
F11
COLO 858 Vemurafenib Palbociclib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 9.05 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 112.47 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 742.22
Colo858-P9
G11
WM-115 Vemurafenib AZD8055 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 786.69 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 112.70 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 219.55
WM115-P9
E6
A-375 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 253.49 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 113.07 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 312.17
A375-P4
G4
WM-115 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 478.06 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 113.15 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 193.46
WM115-P10
C2
WM-115 Vemurafenib AZD8055 1.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 717.49 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 113.19 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 27.56
WM115-P8
G7
A-375 Vemurafenib Trametinib 1.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 143.67 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 113.29 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 389.91
A375-P4
D5
WM-115 Vemurafenib Trametinib 0.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 286.80 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 113.41 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 183.08
WM115-P4
E5
COLO 858 Vemurafenib Defactinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 392.04 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 113.44 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 408.87
Colo858-P2
G7
COLO 858 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 126.67 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 113.51 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 619.72
Colo858-P8
F10
WM-115 Vemurafenib Pictilisib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 538.01 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 114.20 NGFR rabbit IgG(H+L) / donkey NGFR 1201.41
WM115-P7
G4
WM-115 Vemurafenib AZD8055 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 752.21 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 114.35 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 52.89
WM115-P8
E6
WM-115 Vemurafenib Trametinib 1.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 468.25 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 114.36 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 450.42
WM115-P3
G5
WM-115 Vemurafenib Trametinib 1.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 310.66 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 114.38 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 485.74
WM115-P3
D5
WM-115 Vemurafenib JNK-IN-8 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 437.91 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 114.52 NGFR rabbit IgG(H+L) / donkey NGFR 1444.39
WM115-P7
C8
WM-115 Vemurafenib Pictilisib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 527.34 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 114.52 NGFR rabbit IgG(H+L) / donkey NGFR 1693.04
WM115-P7
D4
A-375 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 297.68 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 114.57 NGFR rabbit IgG(H+L) / donkey NGFR 358.16
A375-P7
C11
COLO 858 Vemurafenib Palbociclib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 93.37 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 114.75 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 739.65
Colo858-P9
G10
WM-115 Vemurafenib Defactinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 535.82 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 114.93 NGFR rabbit IgG(H+L) / donkey NGFR 1519.23
WM115-P1
F6