Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 198.08 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 109.01 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 228.77
A375-P4
G8
WM-115 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 339.36 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 109.01 NGFR rabbit IgG(H+L) / donkey NGFR 1247.51
WM115-P7
E10
A-375 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 189.10 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 109.08 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 210.36
A375-P10
F11
WM-115 Vemurafenib JNK-IN-8 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 400.52 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 109.62 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 81.34
WM115-P10
C8
COLO 858 Vemurafenib PF562271 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 342.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 109.69 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 261.12
Colo858-P2
G9
COLO 858 Vemurafenib AZD8055 1.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 72.49 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 109.75 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 309.34
Colo858-P10
D7
WM-115 Vemurafenib Pictilisib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 456.56 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 109.93 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 367.78
WM115-P9
C4
WM-115 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 338.40 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 110.02 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 186.62
WM115-P4
B4
WM-115 Vemurafenib Pictilisib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 427.24 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 110.14 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 191.66
WM115-P10
C4
WM-115 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 334.98 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 110.25 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 108.33
WM115-P4
F11
WM-115 Vemurafenib Trametinib 0.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 426.47 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 110.31 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 473.36
WM115-P3
E5
WM-115 Vemurafenib Trametinib 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 446.29 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 110.40 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 454.27
WM115-P3
F4
COLO 858 Vemurafenib Palbociclib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 69.97 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 110.48 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 396.58
Colo858-P10
D10
A-375 Vemurafenib Palbociclib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 295.35 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 110.52 NGFR rabbit IgG(H+L) / donkey NGFR 408.50
A375-P7
G11
A-375 Vemurafenib Palbociclib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 303.56 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 110.58 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1046.90
A375-P8
D11
WM-115 Vemurafenib Defactinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 436.64 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 110.62 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1937.83
WM115-P2
F7
WM-115 Vemurafenib Trametinib 1.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 324.85 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 110.98 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 568.37
WM115-P2
D5
WM-115 Vemurafenib Dasatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 420.06 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 111.06 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 303.99
WM115-P2
G10
WM-115 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 487.22 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 111.16 NGFR rabbit IgG(H+L) / donkey NGFR 1552.62
WM115-P7
G8
WM-115 Vemurafenib Saracatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 494.80 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 111.31 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 539.47
WM115-P8
D3
COLO 858 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 247.70 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 111.32 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 325.39
Colo858-P4
D8
WM-115 Vemurafenib Pictilisib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 404.40 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 111.36 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 285.66
WM115-P9
C5
WM-115 Vemurafenib PF562271 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 314.55 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 111.45 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 2092.76
WM115-P2
D9
A-375 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 292.25 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 111.45 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 355.00
A375-P9
C10
COLO 858 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 90.47 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 111.48 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 341.30
Colo858-P10
C6