Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib Palbociclib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 77.86 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 60.54 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 326.39
WM115-P9
D11
A-375 Vemurafenib Defactinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 86.86 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 107.46 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 264.66
A375-P4
D6
COLO 858 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 90.47 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 111.48 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 341.30
Colo858-P10
C6
COLO 858 Vemurafenib Palbociclib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 93.37 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 114.75 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 739.65
Colo858-P9
G10
WM-115 Vemurafenib Defactinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 99.01 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 83.20 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 199.43
WM115-P4
D6
WM-115 Vemurafenib Palbociclib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 100.60 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 52.74 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 137.72
WM115-P10
G10
COLO 858 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 104.50 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 119.60 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 587.36
Colo858-P8
F11
WM-115 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 109.09 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 69.20 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 60.22
WM115-P10
B11
WM-115 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 110.85 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 83.69 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 101.74
WM115-P9
B10
WM-115 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 111.38 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 54.11 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 162.53
WM115-P10
F11
WM-115 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 114.25 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 69.04 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 383.22
WM115-P9
F10
COLO 858 Vemurafenib Defactinib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 116.51 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 128.22 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 241.68
Colo858-P4
D6
WM-115 Vemurafenib Palbociclib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 116.60 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 60.05 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 306.18
WM115-P9
G10
A-375 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 118.73 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 92.50 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 196.46
A375-P10
C11
COLO 858 Vemurafenib Palbociclib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 120.14 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 124.94 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 815.68
Colo858-P9
D10
COLO 858 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 120.81 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 106.60 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 344.47
Colo858-P10
F6
WM-115 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 122.04 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 70.76 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1031.66
WM115-P8
C11
WM-115 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 124.55 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 73.03 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 100.03
WM115-P9
E11
COLO 858 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 126.67 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 113.51 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 619.72
Colo858-P8
F10
WM-115 Vemurafenib Defactinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 134.07 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 88.13 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 186.13
WM115-P4
D7
WM-115 Vemurafenib PF562271 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.31 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 73.59 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 89.45
WM115-P4
D9
COLO 858 Vemurafenib Palbociclib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 142.44 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 108.82 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 233.16
Colo858-P8
D11
A-375 Vemurafenib Trametinib 1.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 143.67 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 113.29 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 389.91
A375-P4
D5
COLO 858 Vemurafenib Pictilisib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 146.50 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 149.92 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 238.64
Colo858-P10
D5
COLO 858 Vemurafenib Palbociclib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 146.77 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 106.96 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 257.34
Colo858-P8
G10