Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib PF562271 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 342.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 109.69 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 261.12
Colo858-P2
G9
WM-115 Vemurafenib JNK-IN-8 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 342.51 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 84.72 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 56.65
WM115-P10
F8
WM-115 Vemurafenib 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 343.36 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 96.42 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 225.02
WM115-P4
D3
COLO 858 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 343.47 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 120.07 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 432.43
Colo858-P2
G8
WM-115 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 343.99 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 85.55 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 244.04
WM115-P8
F9
A-375 Vemurafenib Defactinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 344.82 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 136.28 NGFR rabbit IgG(H+L) / donkey NGFR 282.18
A375-P1
D7
WM-115 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 344.87 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 74.07 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 151.25
WM115-P10
G2
COLO 858 Vemurafenib AZD8055 0.316 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 345.13 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 143.25 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 575.18
Colo858-P9
F7
COLO 858 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 345.37 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 149.25 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 684.43
Colo858-P3
D8
WM-115 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 345.84 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 86.32 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 42.90
WM115-P10
F9
COLO 858 Vemurafenib AZD8055 1.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 345.85 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 138.16 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 741.60
Colo858-P9
G7
A-375 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 345.98 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 120.79 NGFR rabbit IgG(H+L) / donkey NGFR 369.72
A375-P7
D8
A-375 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 346.07 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 142.60 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 856.27
A375-P3
D4
WM-115 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 347.86 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 130.76 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 82.98
WM115-P4
B11
A-375 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 348.73 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 117.36 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 318.34
A375-P9
D9
A-375 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 348.98 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 146.33 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 218.98
A375-P4
C10
WM-115 Vemurafenib Saracatinib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 349.41 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 85.72 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 310.39
WM115-P9
D3
A-375 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 349.48 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 133.72 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 67.57
A375-P10
G9
A-375 Vemurafenib PF562271 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 350.60 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 131.47 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 244.17
A375-P3
G9
A-375 Vemurafenib Defactinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 351.44 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 128.21 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 449.65
A375-P3
C7
COLO 858 Vemurafenib PF562271 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 351.99 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 161.36 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 575.58
Colo858-P3
C9
A-375 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 352.46 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 140.18 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 207.24
A375-P4
F10
WM-115 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 353.70 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 108.28 NGFR rabbit IgG(H+L) / donkey NGFR 2500.22
WM115-P1
D8
A-375 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 354.17 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 209.98 NGFR rabbit IgG(H+L) / donkey NGFR 635.87
A375-P7
F10
A-375 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 354.87 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 143.46 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 364.71
A375-P3
C10