Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Trametinib 1.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 386.85 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 146.10 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 798.02
A375-P3
G5
A-375 Vemurafenib Defactinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 379.23 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 124.65 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 402.65
A375-P3
G6
A-375 Vemurafenib Defactinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 391.87 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 132.61 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 397.86
A375-P3
G7
A-375 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 391.19 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 127.30 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 388.14
A375-P3
G8
A-375 Vemurafenib PF562271 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 350.60 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 131.47 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 244.17
A375-P3
G9
COLO 858 Vemurafenib Palbociclib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 93.37 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 114.75 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 739.65
Colo858-P9
G10
COLO 858 Vemurafenib Palbociclib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 9.05 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 112.47 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 742.22
Colo858-P9
G11
COLO 858 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 509.46 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 177.66 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 607.02
Colo858-P9
G2
COLO 858 Vemurafenib Saracatinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 539.47 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 192.62 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 415.11
Colo858-P9
G3
COLO 858 Vemurafenib Pictilisib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 469.06 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 185.49 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 541.20
Colo858-P9
G4
COLO 858 Vemurafenib Pictilisib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 367.86 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 178.35 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 482.06
Colo858-P9
G5
COLO 858 Vemurafenib AZD8055 1.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 449.08 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 183.61 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 748.66
Colo858-P9
G6
COLO 858 Vemurafenib AZD8055 1.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 345.85 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 138.16 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 741.60
Colo858-P9
G7
COLO 858 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 410.15 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 169.53 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 360.95
Colo858-P9
G8
COLO 858 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 392.22 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 165.29 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 238.96
Colo858-P9
G9