Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 682.13 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 193.50 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 718.41
Colo858-P2
D3
COLO 858 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1303.68 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 244.16 NGFR rabbit IgG(H+L) / donkey NGFR 525.63
Colo858-P1
C2
A-375 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 686.02 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 174.00 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 634.81
A375-P3
C3
A-375 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 770.48 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 175.13 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 660.50
A375-P3
C2
COLO 858 Vemurafenib 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 676.04 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 171.31 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 406.66
Colo858-P4
G3
COLO 858 Vemurafenib 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 694.26 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 360.82 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 347.86
Colo858-P4
G2
COLO 858 Vemurafenib 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 957.68 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 217.74 NGFR rabbit IgG(H+L) / donkey NGFR 769.46
Colo858-P1
D2
A-375 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3094.28 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 552.89 NGFR rabbit IgG(H+L) / donkey NGFR 593.97
A375-P1
B2
COLO 858 Vemurafenib 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 807.64 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 196.69 NGFR rabbit IgG(H+L) / donkey NGFR 771.35
Colo858-P1
D3
WM-115 Vemurafenib 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 615.31 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 134.00 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 822.24
WM115-P2
D2
WM-115 Vemurafenib 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 513.28 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 126.53 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 852.13
WM115-P2
D3
COLO 858 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2329.97 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 322.63 NGFR rabbit IgG(H+L) / donkey NGFR 125.82
Colo858-P1
B2
A-375 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2544.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 399.02 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 453.70
A375-P3
B3
A-375 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2682.85 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 414.64 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 436.39
A375-P3
B2
COLO 858 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2508.82 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 298.52 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 135.52
Colo858-P3
E2