Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1145.34 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 202.17 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 96.67
Colo858-P10
B10
COLO 858 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3589.10 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 482.80 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 7315.73
Colo858-P2
B10
COLO 858 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3822.30 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 483.25 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 109.03
Colo858-P3
B10
COLO 858 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3881.18 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 466.69 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 124.41
Colo858-P4
B10
COLO 858 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1923.42 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 239.88 NGFR rabbit IgG(H+L) / donkey NGFR 87.10
Colo858-P7
B10
COLO 858 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1108.51 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 248.16 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 4704.55
Colo858-P8
B10
A-375 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2722.08 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 510.86 NGFR rabbit IgG(H+L) / donkey NGFR 141.68
A375-P1
B10
WM-115 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 734.28 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 203.21 NGFR rabbit IgG(H+L) / donkey NGFR 624.02
WM115-P1
B10
WM-115 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 157.69 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 89.34 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 67.20
WM115-P10
B10
WM-115 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 693.46 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 203.81 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 2535.44
WM115-P2
B10
WM-115 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 667.67 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 186.16 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 112.50
WM115-P3
B10
WM-115 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 475.83 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 162.62 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 83.55
WM115-P4
B10
WM-115 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 319.14 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 118.20 NGFR rabbit IgG(H+L) / donkey NGFR 1378.93
WM115-P7
B10
WM-115 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 177.77 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 107.45 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 2909.88
WM115-P8
B10
WM-115 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 110.85 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 83.69 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 101.74
WM115-P9
B10