Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

 Full screen view
Filter results: >>>>
Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 457.50 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 119.63 NGFR rabbit IgG(H+L) / donkey NGFR 310.49
A375-P7
G8
COLO 858 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 410.15 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 169.53 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 360.95
Colo858-P9
G8
A-375 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 487.81 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 153.46 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 583.71
A375-P8
G8
COLO 858 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 407.94 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 146.43 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 634.29
Colo858-P3
G8
A-375 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 448.60 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 136.13 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 635.30
A375-P2
G8
WM-115 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 313.29 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 78.88 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 452.14
WM115-P9
G8
WM-115 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 363.91 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 79.92 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 368.38
WM115-P8
G8
A-375 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 391.19 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 127.30 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 388.14
A375-P3
G8
WM-115 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 423.89 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 108.70 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 368.15
WM115-P3
G8
COLO 858 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1152.85 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 197.53 NGFR rabbit IgG(H+L) / donkey NGFR 216.41
Colo858-P7
G8
WM-115 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 235.11 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 87.45 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 154.60
WM115-P4
G8
A-375 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 522.12 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 150.15 NGFR rabbit IgG(H+L) / donkey NGFR 600.07
A375-P1
G8
A-375 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 198.08 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 109.01 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 228.77
A375-P4
G8
COLO 858 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 343.47 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 120.07 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 432.43
Colo858-P2
G8
A-375 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 283.15 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 118.66 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 82.01
A375-P10
G8
WM-115 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 447.94 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 119.74 NGFR rabbit IgG(H+L) / donkey NGFR 2263.76
WM115-P1
G8
COLO 858 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 529.15 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 153.33 NGFR rabbit IgG(H+L) / donkey NGFR 402.57
Colo858-P1
G8
COLO 858 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 372.17 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 119.07 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 261.05
Colo858-P4
G8
WM-115 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 378.45 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 97.15 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1106.45
WM115-P2
G8
COLO 858 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 300.94 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 147.84 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 85.73
Colo858-P10
G8
A-375 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 326.16 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 102.57 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 297.95
A375-P9
G8
WM-115 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 301.22 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 77.74 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 48.25
WM115-P10
G8
WM-115 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 487.22 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 111.16 NGFR rabbit IgG(H+L) / donkey NGFR 1552.62
WM115-P7
G8
A-375 Vemurafenib Defactinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 395.53 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 136.58 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 257.47
A375-P4
G7
A-375 Vemurafenib Defactinib 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 391.87 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 132.61 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 397.86
A375-P3
G7