Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Dasatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 476.42 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 172.28 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1134.30
A375-P2
C10
A-375 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 539.06 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 158.41 NGFR rabbit IgG(H+L) / donkey NGFR 1132.49
A375-P1
E4
COLO 858 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 876.70 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 214.24 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1131.61
Colo858-P2
C3
WM-115 Vemurafenib Pictilisib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 644.23 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 167.52 NGFR rabbit IgG(H+L) / donkey NGFR 1131.58
WM115-P7
C5
A-375 Vemurafenib Trametinib 0.316 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 463.41 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 158.22 NGFR rabbit IgG(H+L) / donkey NGFR 1124.92
A375-P1
C5
WM-115 Vemurafenib Pictilisib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 638.63 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 149.51 NGFR rabbit IgG(H+L) / donkey NGFR 1122.90
WM115-P7
E4
WM-115 Vemurafenib Pictilisib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 557.45 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 131.39 NGFR rabbit IgG(H+L) / donkey NGFR 1122.76
WM115-P7
D5
COLO 858 Vemurafenib Trametinib 1.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 582.43 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 155.15 NGFR rabbit IgG(H+L) / donkey NGFR 1119.00
Colo858-P1
D4
WM-115 Vemurafenib Pictilisib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 638.24 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 156.07 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1118.36
WM115-P8
B4
WM-115 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1228.57 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 363.06 NGFR rabbit IgG(H+L) / donkey NGFR 1118.25
WM115-P7
B2
COLO 858 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1073.52 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 215.52 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1115.43
Colo858-P2
C2
COLO 858 Vemurafenib Trametinib 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 613.16 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 166.35 NGFR rabbit IgG(H+L) / donkey NGFR 1111.88
Colo858-P1
E4
WM-115 Vemurafenib PF562271 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 378.45 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 97.15 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1106.45
WM115-P2
G8
COLO 858 Vemurafenib Trametinib 0.316 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 572.16 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 165.44 NGFR rabbit IgG(H+L) / donkey NGFR 1097.04
Colo858-P1
C5
WM-115 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 914.21 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 249.27 NGFR rabbit IgG(H+L) / donkey NGFR 1096.13
WM115-P7
E8
COLO 858 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 896.62 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 199.86 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1095.29
Colo858-P2
F2
A-375 Vemurafenib JNK-IN-8 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 434.21 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 153.22 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1093.74
A375-P8
C8
COLO 858 Vemurafenib Defactinib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 570.66 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 174.34 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1093.29
Colo858-P2
F6
A-375 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 593.31 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 155.33 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1088.62
A375-P8
C2
A-375 Vemurafenib Trametinib 1.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 555.54 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 165.74 NGFR rabbit IgG(H+L) / donkey NGFR 1086.87
A375-P1
G5
COLO 858 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 610.87 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 183.14 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1075.09
Colo858-P2
C11
COLO 858 Vemurafenib Trametinib 0.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 562.03 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 167.50 NGFR rabbit IgG(H+L) / donkey NGFR 1064.46
Colo858-P1
E5
COLO 858 Vemurafenib Palbociclib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 820.67 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 137.82 NGFR rabbit IgG(H+L) / donkey NGFR 1062.99
Colo858-P7
G10
A-375 Vemurafenib AZD8055 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1459.47 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 335.75 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1057.98
A375-P8
E6
WM-115 Vemurafenib Defactinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 413.87 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 101.67 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1049.83
WM115-P2
G6