Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib AZD8055 0.316 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 653.50 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 78.35 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 490.17
WM115-P9
F6
WM-115 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 301.22 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 77.74 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 48.25
WM115-P10
G8
WM-115 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 195.63 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 77.41 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 65.04
WM115-P10
E10
WM-115 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 207.71 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 77.05 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 513.72
WM115-P9
D8
WM-115 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 290.72 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 76.87 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 183.75
WM115-P8
D9
WM-115 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 170.14 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 76.83 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 109.85
WM115-P9
E10
WM-115 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 173.45 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 75.38 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1286.81
WM115-P8
C10
WM-115 Vemurafenib Palbociclib 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 186.36 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 74.65 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 642.16
WM115-P8
D10
WM-115 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 194.59 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 74.59 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1212.19
WM115-P8
F10
WM-115 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 344.87 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 74.07 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 151.25
WM115-P10
G2
WM-115 Vemurafenib Palbociclib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 155.14 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 73.93 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 692.64
WM115-P8
G10
WM-115 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 164.67 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 73.89 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 62.75
WM115-P10
E11
WM-115 Vemurafenib PF562271 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 140.31 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 73.59 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 89.45
WM115-P4
D9
WM-115 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 365.31 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 73.56 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 434.05
WM115-P8
G2
WM-115 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 294.05 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 73.04 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 351.08
WM115-P9
G9
WM-115 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 124.55 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 73.03 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 100.03
WM115-P9
E11
WM-115 Vemurafenib Palbociclib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 122.04 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 70.76 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 1031.66
WM115-P8
C11
WM-115 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 156.27 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 70.38 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 196.20
WM115-P10
C10
WM-115 Vemurafenib JNK-IN-8 1.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 216.47 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 69.53 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 334.55
WM115-P8
D8
WM-115 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 150.21 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 69.37 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 398.64
WM115-P9
C10
WM-115 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 109.09 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 69.20 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 60.22
WM115-P10
B11
WM-115 Vemurafenib Palbociclib 0.316 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 114.25 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 69.04 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 383.22
WM115-P9
F10
WM-115 Vemurafenib Palbociclib 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 159.56 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 67.99 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 645.54
WM115-P8
D11
WM-115 Vemurafenib JNK-IN-8 1.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 268.33 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 67.53 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 359.09
WM115-P9
D9
WM-115 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 56.24 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 66.25 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 93.13
WM115-P9
B11