Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
A-375 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1854.98 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 413.60 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 7390.58
A375-P2
B11
A-375 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2096.28 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 413.41 NGFR rabbit IgG(H+L) / donkey NGFR 143.89
A375-P7
B9
A-375 Vemurafenib Pictilisib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2012.07 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 413.37 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 5072.92
A375-P8
B4
COLO 858 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3490.89 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 412.36 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 99.67
Colo858-P4
B11
A-375 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2565.82 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 412.30 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 260.81
A375-P4
E3
COLO 858 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3266.62 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 411.78 NGFR rabbit IgG(H+L) / donkey NGFR 137.05
Colo858-P7
B9
A-375 Vemurafenib Pictilisib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2053.10 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 410.35 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 4952.05
A375-P8
E4
COLO 858 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2604.12 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 409.44 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 5110.94
Colo858-P8
E3
COLO 858 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2571.01 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 408.55 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 5297.17
Colo858-P8
B9
A-375 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2413.32 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 408.16 NGFR rabbit IgG(H+L) / donkey NGFR 329.47
A375-P7
E8
A-375 Vemurafenib Dasatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2181.70 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 406.40 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 169.81
A375-P4
B10
A-375 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2223.28 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 406.34 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 214.00
A375-P10
E2
A-375 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2231.52 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 405.65 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 204.22
A375-P10
E3
COLO 858 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3383.30 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 405.53 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 96.01
Colo858-P3
B11
A-375 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2597.12 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 404.96 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 265.99
A375-P4
B2
A-375 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2249.34 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 403.96 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 236.71
A375-P9
B3
A-375 Vemurafenib Defactinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1907.41 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 403.84 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 7872.32
A375-P2
B7
COLO 858 Vemurafenib Dasatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3107.17 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 403.20 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 5963.42
Colo858-P2
B11
A-375 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2735.24 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 403.13 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 434.01
A375-P3
E2
A-375 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2084.99 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 403.10 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 190.22
A375-P10
B2
A-375 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2623.48 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 402.51 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 432.33
A375-P3
E3
COLO 858 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3190.16 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 401.49 NGFR rabbit IgG(H+L) / donkey NGFR 133.47
Colo858-P7
E9
COLO 858 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2609.81 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 401.06 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 4949.40
Colo858-P8
B3
COLO 858 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3260.79 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 400.81 NGFR rabbit IgG(H+L) / donkey NGFR 142.45
Colo858-P7
B2
COLO 858 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 3359.90 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 399.82 NGFR rabbit IgG(H+L) / donkey NGFR 133.84
Colo858-P7
B3