Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 816.83 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 243.30 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 4483.57
Colo858-P8
B11
COLO 858 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2022.74 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 242.98 NGFR rabbit IgG(H+L) / donkey NGFR 131.68
Colo858-P7
B7
COLO 858 Vemurafenib AZD8055 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2264.46 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 242.71 NGFR rabbit IgG(H+L) / donkey NGFR 101.71
Colo858-P7
B6
COLO 858 Vemurafenib AZD8055 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1578.35 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 242.71 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 212.87
Colo858-P9
B6
COLO 858 Vemurafenib Defactinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1665.28 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 240.80 NGFR rabbit IgG(H+L) / donkey NGFR 99.42
Colo858-P1
E7
COLO 858 Vemurafenib Dasatinib 0.316 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 873.02 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 240.79 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 169.62
Colo858-P3
C11
COLO 858 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1923.42 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 239.88 NGFR rabbit IgG(H+L) / donkey NGFR 87.10
Colo858-P7
B10
COLO 858 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1921.89 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 238.82 NGFR rabbit IgG(H+L) / donkey NGFR 86.63
Colo858-P7
E10
WM-115 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1139.55 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 238.74 NGFR rabbit IgG(H+L) / donkey NGFR 1292.52
WM115-P1
E3
WM-115 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1023.11 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 238.66 NGFR rabbit IgG(H+L) / donkey NGFR 1240.61
WM115-P1
B3
COLO 858 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1183.13 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 238.18 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 4614.75
Colo858-P8
E10
COLO 858 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1339.44 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 236.98 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 267.54
Colo858-P9
B10
COLO 858 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1181.89 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 235.96 NGFR rabbit IgG(H+L) / donkey NGFR 475.07
Colo858-P1
C3
COLO 858 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 803.08 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 235.00 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 447.38
Colo858-P8
C2
COLO 858 Vemurafenib Defactinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1846.39 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 234.83 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 111.79
Colo858-P4
B7
A-375 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1042.51 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 234.63 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 2631.95
A375-P2
F2
WM-115 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 885.39 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 234.32 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 206.30
WM115-P9
E8
WM-115 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 989.36 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 234.08 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 3304.49
WM115-P2
E3
COLO 858 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1188.52 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 232.45 NGFR rabbit IgG(H+L) / donkey NGFR 555.83
Colo858-P1
F2
COLO 858 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 828.41 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 231.09 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 648.00
Colo858-P9
C2
A-375 Vemurafenib JNK-IN-8 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 684.84 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 231.07 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 929.36
A375-P8
F9
COLO 858 Vemurafenib AZD8055 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1564.84 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 230.30 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 166.92
Colo858-P9
E6
A-375 Vemurafenib 0.316
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1022.20 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 229.98 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 2502.46
A375-P2
F3
WM-115 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 849.20 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 229.62 NGFR rabbit IgG(H+L) / donkey NGFR 923.15
WM115-P7
E9
A-375 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1112.72 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 228.94 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 6005.37
A375-P8
E10