Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
COLO 858 Vemurafenib Defactinib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1753.37 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 270.91 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 4904.27
Colo858-P2
B7
A-375 Vemurafenib Defactinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1330.25 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 270.77 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 275.47
A375-P3
E7
COLO 858 Vemurafenib Defactinib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2379.05 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 269.47 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 102.93
Colo858-P4
B6
A-375 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1214.84 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 269.03 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 176.94
A375-P10
B7
COLO 858 Vemurafenib Defactinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1765.19 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 268.42 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 283.16
Colo858-P3
E7
COLO 858 Vemurafenib Defactinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2330.81 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 267.45 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 94.06
Colo858-P4
E6
A-375 Vemurafenib Pictilisib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1121.62 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 267.34 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 152.07
A375-P10
E5
COLO 858 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2310.31 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 267.07 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 112.58
Colo858-P4
E2
COLO 858 Vemurafenib Saracatinib 1.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1476.11 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 266.46 NGFR rabbit IgG(H+L) / donkey NGFR 426.26
Colo858-P7
G2
A-375 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1278.24 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 266.10 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 287.39
A375-P9
B7
COLO 858 Vemurafenib PF562271 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 2338.10 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 265.73 c-Jun (pS73) rabbit IgG(H+L) / donkey c-Jun (pS73) 100.51
Colo858-P4
E8
A-375 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1069.24 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 259.50 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 7515.65
A375-P2
E9
WM-115 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 906.98 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 259.11 NGFR rabbit IgG(H+L) / donkey NGFR 1044.06
WM115-P7
B9
A-375 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1304.14 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 256.23 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 279.80
A375-P9
E7
COLO 858 Vemurafenib Saracatinib 0.316 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1575.75 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 255.59 NGFR rabbit IgG(H+L) / donkey NGFR 300.64
Colo858-P7
C2
WM-115 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 942.28 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 253.64 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 3240.91
WM115-P8
B8
COLO 858 Vemurafenib Saracatinib 0.316 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1473.47 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 253.19 NGFR rabbit IgG(H+L) / donkey NGFR 127.22
Colo858-P7
F3
A-375 Vemurafenib PF562271 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1339.47 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 253.18 NGFR rabbit IgG(H+L) / donkey NGFR 154.21
A375-P1
B9
A-375 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1069.82 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 249.87 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 2417.92
A375-P2
C2
A-375 Vemurafenib Pictilisib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1133.62 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 249.77 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 233.49
A375-P9
E5
WM-115 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 914.21 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 249.27 NGFR rabbit IgG(H+L) / donkey NGFR 1096.13
WM115-P7
E8
WM-115 Vemurafenib 0.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1159.99 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 248.75 NGFR rabbit IgG(H+L) / donkey NGFR 1290.83
WM115-P1
E2
COLO 858 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1108.51 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 248.16 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 4704.55
Colo858-P8
B10
WM-115 Vemurafenib 0.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1083.20 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 246.45 S6 (pS240; pS244) rabbit IgG(H+L) / donkey S6 (pS240; pS244) 3292.54
WM115-P2
B2
COLO 858 Vemurafenib 0.316
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 1303.68 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 244.16 NGFR rabbit IgG(H+L) / donkey NGFR 525.63
Colo858-P1
C2