Phosphorylation state and protein levels measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of two compounds (immunofluorescence dataset 1 of 4) - Dataset (ID:20274)

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Cell Small Molecule 1 Small Molecule 2 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Primary Antibody (488) Secondary Antibody (488) Protein Detected (488) Mean Fluorescence Intensity (488) Primary Antibody (568) Secondary Antibody (568) Protein Detected (568) Mean Fluorescence Intensity (568) Primary Antibody (647) Secondary Antibody (647) Protein Detected (647) Mean Fluorescence Intensity (647) Plate Name Plate Well
WM-115 Vemurafenib Pictilisib 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 499.81 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 115.87 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 146.91
WM115-P9
B5
WM-115 Vemurafenib AZD8055 0.000 0.200
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 915.31 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 126.86 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 227.18
WM115-P9
B6
WM-115 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 847.88 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 143.75 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 204.30
WM115-P9
B7
WM-115 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 821.74 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 214.74 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 180.19
WM115-P9
B8
WM-115 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 1 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 705.77 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 198.34 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 186.90
WM115-P9
B9
WM-115 Vemurafenib Palbociclib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 170.14 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 76.83 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 109.85
WM115-P9
E10
WM-115 Vemurafenib Palbociclib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 124.55 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 73.03 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 100.03
WM115-P9
E11
WM-115 Vemurafenib Saracatinib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 712.23 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 152.78 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 136.83
WM115-P9
E2
WM-115 Vemurafenib Saracatinib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 652.14 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 155.11 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 124.96
WM115-P9
E3
WM-115 Vemurafenib Pictilisib 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 489.76 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 119.68 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 183.88
WM115-P9
E4
WM-115 Vemurafenib Pictilisib 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 425.66 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 107.69 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 162.12
WM115-P9
E5
WM-115 Vemurafenib AZD8055 0.000 0.200
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 786.69 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 112.70 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 219.55
WM115-P9
E6
WM-115 Vemurafenib AZD8055 0.000 0.600
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 882.34 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 141.39 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 210.67
WM115-P9
E7
WM-115 Vemurafenib JNK-IN-8 0.000 1.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 885.39 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 234.32 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 206.30
WM115-P9
E8
WM-115 Vemurafenib JNK-IN-8 0.000 3.000
uM
 48
hr
 2 KI-67 / clone 8D5 mouse IgG(H+L) / donkey KI-67 603.17 Rb (pS807; pS811) goat IgG(H+L) Rb (pS807; pS811) 174.63 c-Jun / clone 60A8 rabbit IgG(H+L) / donkey c-Jun 186.80
WM115-P9
E9