Viability and apoptosis measured by imaging in BRAF(V600E/D) melanoma cell lines following treatment with combinations of three compounds (viability/apoptosis dataset 2 of 2) - Dataset (ID:20273)

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Cell Small Molecule 1 Small Molecule 2 Small Molecule 3 Small Mol 1 Concentration Small Mol 2 Concentration Small Mol 3 Concentration Small Mol Conc Unit Time Point Time Point Unit Replicate Total Number of Cells (Nuclei) Number of Apoptotic Cells Relative Viability Mean Relative Viability Apoptosis Fraction Mean Apoptosis Fraction
COLO 858 Vemurafenib Trametinib Dasatinib 3.1600 0.3160 1.0000
uM
 72
hr
 1 1387 1358 0.0048 0.0049 0.9791 0.9743
COLO 858 Vemurafenib Trametinib Saracatinib 3.1600 0.3160 1.0000
uM
 72
hr
 2 1171 1145 0.0044 0.0051 0.9778 0.9739
COLO 858 Vemurafenib Trametinib Dasatinib 1.0000 0.1000 1.0000
uM
 72
hr
 2 1083 1059 0.0040 0.0044 0.9778 0.9751
COLO 858 Vemurafenib Trametinib Dasatinib 3.1600 0.3160 1.0000
uM
 72
hr
 2 1237 1209 0.0046 0.0049 0.9774 0.9743
COLO 858 Vemurafenib Trametinib Dasatinib 3.1600 0.3160 3.1600
uM
 72
hr
 3 975 952 0.0038 0.0030 0.9764 0.9776
COLO 858 Vemurafenib Trametinib Dasatinib 3.1600 0.3160 3.1600
uM
 72
hr
 2 717 700 0.0028 0.0030 0.9763 0.9776
COLO 858 Vemurafenib Trametinib Saracatinib 3.1600 0.3160 1.0000
uM
 72
hr
 3 929 907 0.0037 0.0051 0.9763 0.9739
COLO 858 Vemurafenib Trametinib Dasatinib 1.0000 0.1000 0.3160
uM
 72
hr
 2 712 695 0.0028 0.0029 0.9761 0.9759
COLO 858 Vemurafenib Trametinib Dasatinib 0.1000 0.0100 0.3160
uM
 72
hr
 3 1204 1175 0.0048 0.0045 0.9759 0.9731
COLO 858 Vemurafenib Trametinib Dasatinib 0.1000 0.0100 0.3160
uM
 72
hr
 1 849 828 0.0035 0.0045 0.9753 0.9731
COLO 858 Vemurafenib Trametinib PF562271 3.1600 0.3160 0.3160
uM
 72
hr
 3 687 670 0.0033 0.0068 0.9753 0.9483
COLO 858 Vemurafenib Trametinib Dasatinib 3.1600 0.3160 0.3160
uM
 72
hr
 2 755 735 0.0033 0.0020 0.9735 0.9824
COLO 858 Vemurafenib Trametinib Saracatinib 3.1600 0.3160 3.1600
uM
 72
hr
 1 817 795 0.0037 0.0038 0.9731 0.9738
COLO 858 Vemurafenib Trametinib Dasatinib 0.3160 0.0316 0.3160
uM
 72
hr
 1 668 650 0.0030 0.0042 0.9731 0.9671
COLO 858 Vemurafenib Trametinib Dasatinib 1.0000 0.1000 0.3160
uM
 72
hr
 1 618 601 0.0028 0.0029 0.9725 0.9759
COLO 858 Vemurafenib Trametinib PF562271 3.1600 0.3160 1.0000
uM
 72
hr
 2 1075 1043 0.0062 0.0091 0.9702 0.9558
COLO 858 Vemurafenib Trametinib Dasatinib 0.1000 0.0100 0.3160
uM
 72
hr
 2 975 944 0.0051 0.0045 0.9682 0.9731
COLO 858 Vemurafenib Trametinib Dasatinib 1.0000 0.1000 1.0000
uM
 72
hr
 3 1006 974 0.0053 0.0044 0.9682 0.9751
COLO 858 Vemurafenib Trametinib Saracatinib 3.1600 0.3160 1.0000
uM
 72
hr
 1 1361 1317 0.0074 0.0051 0.9677 0.9739
COLO 858 Vemurafenib Trametinib Dasatinib 3.1600 0.3160 1.0000
uM
 72
hr
 3 923 892 0.0051 0.0049 0.9664 0.9743
COLO 858 Vemurafenib Trametinib Dasatinib 0.3160 0.0316 0.3160
uM
 72
hr
 2 788 761 0.0045 0.0042 0.9657 0.9671
COLO 858 Vemurafenib Trametinib Saracatinib 3.1600 0.3160 3.1600
uM
 72
hr
 3 917 885 0.0054 0.0038 0.9651 0.9738
COLO 858 Vemurafenib Trametinib (+)-JQ1 1.0000 0.1000 1.0000
uM
 72
hr
 2 756 728 0.0080 0.0118 0.9630 0.9447
COLO 858 Vemurafenib Trametinib I-BET 3.1600 0.3160 1.0000
uM
 72
hr
 3 620 597 0.0065 0.0086 0.9629 0.9481
COLO 858 Vemurafenib Trametinib Dasatinib 1.0000 0.1000 3.1600
uM
 72
hr
 1 754 726 0.0046 0.0058 0.9629 0.9563