Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| JNK-IN-8 | COLO 858 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 450.18 | p-Rb(S807/S811) | 173.59 | p-ERK(T202/Y204) | 691.16 | 1 | Colo858-signal1 | D6 | |
| JNK-IN-8 | COLO 858 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 430.28 | p-Rb(S807/S811) | 171.77 | p-ERK(T202/Y204) | 673.40 | 1 | Colo858-signal1 | D7 | |
| JNK-IN-8 | COLO 858 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 439.43 | p-Rb(S807/S811) | 169.34 | p-ERK(T202/Y204) | 657.25 | 1 | Colo858-signal1 | D8 | |
| JNK-IN-8 | COLO 858 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 436.36 | p-Rb(S807/S811) | 155.67 | p-ERK(T202/Y204) | 648.15 | 1 | Colo858-signal1 | D9 | |
| JNK-IN-8 | COLO 858 | 0.0033 | uM | 24 | hr | | p27 Kip1 | 442.59 | p-Rb(S807/S811) | 160.97 | p-ERK(T202/Y204) | 615.32 | 2 | Colo858-signal1 | E10 | |
| JNK-IN-8 | COLO 858 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 445.15 | p-Rb(S807/S811) | 158.36 | p-ERK(T202/Y204) | 601.12 | 2 | Colo858-signal1 | E11 | |
| JNK-IN-8 | COLO 858 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 839.15 | p-Rb(S807/S811) | 256.43 | p-ERK(T202/Y204) | 789.95 | 2 | Colo858-signal1 | E2 | |
| JNK-IN-8 | COLO 858 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 594.04 | p-Rb(S807/S811) | 231.49 | p-ERK(T202/Y204) | 724.41 | 2 | Colo858-signal1 | E3 | |
| JNK-IN-8 | COLO 858 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 504.81 | p-Rb(S807/S811) | 190.17 | p-ERK(T202/Y204) | 719.29 | 2 | Colo858-signal1 | E4 | |
| JNK-IN-8 | COLO 858 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 445.16 | p-Rb(S807/S811) | 182.28 | p-ERK(T202/Y204) | 673.10 | 2 | Colo858-signal1 | E5 | |
| JNK-IN-8 | COLO 858 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 429.89 | p-Rb(S807/S811) | 170.46 | p-ERK(T202/Y204) | 675.31 | 2 | Colo858-signal1 | E6 | |
| JNK-IN-8 | COLO 858 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 429.12 | p-Rb(S807/S811) | 167.32 | p-ERK(T202/Y204) | 661.59 | 2 | Colo858-signal1 | E7 | |
| JNK-IN-8 | COLO 858 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 437.19 | p-Rb(S807/S811) | 168.12 | p-ERK(T202/Y204) | 644.13 | 2 | Colo858-signal1 | E8 | |
| JNK-IN-8 | COLO 858 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 438.93 | p-Rb(S807/S811) | 156.13 | p-ERK(T202/Y204) | 620.57 | 2 | Colo858-signal1 | E9 | |
| JNK-IN-8 | COLO 858 | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 302.14 | p-Rb(S807/S811) | 172.24 | p-S6(S235/S236 | 407.88 | 1 | Colo858-signal2 | D7 | |
| JNK-IN-8 | COLO 858 | 0.0033 | uM | 24 | hr | | p27 Kip1 | 438.30 | p-Rb(S807/S811) | 160.10 | p-ERK(T202/Y204) | 626.02 | 1 | Colo858-signal1 | D10 | |
| JNK-IN-8 | COLO 858 | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 293.86 | p-Rb(S807/S811) | 167.77 | p-S6(S235/S236 | 396.05 | 1 | Colo858-signal2 | D9 | |
| JNK-IN-8 | COLO 858 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 454.18 | p-Rb(S807/S811) | 159.65 | p-ERK(T202/Y204) | 628.56 | 1 | Colo858-signal1 | D11 | |
| JNK-IN-8 | COLO 858 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 370.03 | p-Rb(S807/S811) | 260.57 | p-S6(S235/S236 | 614.60 | 2 | Colo858-signal2 | E2 | |
| JNK-IN-8 | COLO 858 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 738.89 | p-Rb(S807/S811) | 249.29 | p-ERK(T202/Y204) | 766.04 | 1 | Colo858-signal1 | D2 | |
| JNK-IN-8 | COLO 858 | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 343.78 | p-Rb(S807/S811) | 196.27 | p-S6(S235/S236 | 463.89 | 2 | Colo858-signal2 | E4 | |
| JNK-IN-8 | COLO 858 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 596.02 | p-Rb(S807/S811) | 233.59 | p-ERK(T202/Y204) | 707.32 | 1 | Colo858-signal1 | D3 | |
| JNK-IN-8 | COLO 858 | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 373.77 | p-Rb(S807/S811) | 163.58 | p-S6(S235/S236 | 386.62 | 2 | Colo858-signal2 | E9 | |
| JNK-IN-8 | COLO 858 | 0.0082 | uM | 24 | hr | | Ki-67 | 2226.10 | p-Rb(S807/S811) | 150.62 | p-cJun(S73) | 29.95 | 1 | Colo858-signal3 | D9 | |
| JNK-IN-8 | COLO 858 | 0.0205 | uM | 24 | hr | | Ki-67 | 2267.30 | p-Rb(S807/S811) | 156.38 | p-cJun(S73) | 31.07 | 1 | Colo858-signal3 | D8 |