Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| JNK-IN-8 | COLO 858 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 453.72 | p-Rb(S807/S811) | 173.99 | p-ERK(T202/Y204) | 686.67 | 1 | Colo858-signal1 | D5 | |
| Vemurafenib | COLO 858 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 1101.80 | p-Rb(S807/S811) | 48.19 | p-ERK(T202/Y204) | 9.79 | 1 | Colo858-signal1 | B2 | |
| JNK-IN-8 | COLO 858 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 450.18 | p-Rb(S807/S811) | 173.59 | p-ERK(T202/Y204) | 691.16 | 1 | Colo858-signal1 | D6 | |
| Vemurafenib | COLO 858 | 0.0000 | uM | 24 | hr | | Ki-67 | 2209.00 | p-Rb(S807/S811) | 151.07 | p-cJun(S73) | 28.62 | 1 | Colo858-signal3 | B11 | |
| JNK-IN-8 | COLO 858 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 430.28 | p-Rb(S807/S811) | 171.77 | p-ERK(T202/Y204) | 673.40 | 1 | Colo858-signal1 | D7 | |
| Vemurafenib | COLO 858 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 849.44 | p-Rb(S807/S811) | 79.57 | p-ERK(T202/Y204) | 75.64 | 1 | Colo858-signal1 | B6 | |
| JNK-IN-8 | COLO 858 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 439.43 | p-Rb(S807/S811) | 169.34 | p-ERK(T202/Y204) | 657.25 | 1 | Colo858-signal1 | D8 | |
| Vemurafenib | COLO 858 | 0.1280 | uM | 24 | hr | | Ki-67 | 972.60 | p-Rb(S807/S811) | 76.98 | p-cJun(S73) | 64.88 | 1 | Colo858-signal3 | B6 | |
| JNK-IN-8 | COLO 858 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 436.36 | p-Rb(S807/S811) | 155.67 | p-ERK(T202/Y204) | 648.15 | 1 | Colo858-signal1 | D9 | |
| Vemurafenib | COLO 858 | 0.0033 | uM | 24 | hr | | p-Histone H3(S10) | 326.58 | p-Rb(S807/S811) | 162.30 | p-S6(S235/S236 | 402.84 | 1 | Colo858-signal2 | B10 | |
| Vemurafenib | COLO 858 | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 358.86 | p-Rb(S807/S811) | 155.99 | p-S6(S235/S236 | 377.96 | 1 | Colo858-signal2 | B11 | |
| Vemurafenib | COLO 858 | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 265.46 | p-Rb(S807/S811) | 66.87 | p-S6(S235/S236 | 14.48 | 1 | Colo858-signal2 | B2 | |
| Vemurafenib | COLO 858 | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 163.91 | p-Rb(S807/S811) | 54.08 | p-S6(S235/S236 | 23.01 | 1 | Colo858-signal2 | B3 | |
| Vemurafenib | COLO 858 | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 165.33 | p-Rb(S807/S811) | 58.60 | p-S6(S235/S236 | 48.12 | 1 | Colo858-signal2 | B4 | |
| Vemurafenib | COLO 858 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 144.45 | p-Rb(S807/S811) | 54.34 | p-S6(S235/S236 | 77.03 | 1 | Colo858-signal2 | B5 | |
| Vemurafenib | COLO 858 | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 204.59 | p-Rb(S807/S811) | 83.78 | p-S6(S235/S236 | 220.01 | 1 | Colo858-signal2 | B6 | |
| Vemurafenib | COLO 858 | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 284.31 | p-Rb(S807/S811) | 139.79 | p-S6(S235/S236 | 349.95 | 1 | Colo858-signal2 | B7 | |
| Vemurafenib | COLO 858 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 307.92 | p-Rb(S807/S811) | 162.01 | p-S6(S235/S236 | 403.15 | 1 | Colo858-signal2 | B8 | |
| Vemurafenib | COLO 858 | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | 341.90 | p-Rb(S807/S811) | 169.94 | p-S6(S235/S236 | 414.55 | 1 | Colo858-signal2 | B9 | |
| JNK-IN-8 | COLO 858 | 0.0000 | uM | 24 | hr | | p-Histone H3(S10) | 323.83 | p-Rb(S807/S811) | 165.88 | p-S6(S235/S236 | 385.36 | 1 | Colo858-signal2 | D11 | |
| Vemurafenib | COLO 858 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 496.15 | p-Rb(S807/S811) | 149.24 | p-ERK(T202/Y204) | 495.70 | 1 | Colo858-signal1 | B8 | |
| JNK-IN-8 | COLO 858 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 367.65 | p-Rb(S807/S811) | 192.54 | p-S6(S235/S236 | 440.93 | 1 | Colo858-signal2 | D5 | |
| JNK-IN-8 | COLO 858 | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 302.14 | p-Rb(S807/S811) | 172.24 | p-S6(S235/S236 | 407.88 | 1 | Colo858-signal2 | D7 | |
| JNK-IN-8 | COLO 858 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 288.25 | p-Rb(S807/S811) | 170.31 | p-S6(S235/S236 | 401.43 | 1 | Colo858-signal2 | D8 | |
| JNK-IN-8 | COLO 858 | 0.8000 | uM | 24 | hr | | Ki-67 | 2383.20 | p-Rb(S807/S811) | 179.73 | p-cJun(S73) | 31.88 | 1 | Colo858-signal3 | D4 |