Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | WM-115 | JNK-IN-8 | 0.1280 | uM | 24 | hr | | Ki-67 | 779.71 | p-Rb(S807/S811) | 58.29 | p-cJun(S73) | 34.35 | 2 | WM115-signal3 | G6 |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.1280 | uM | 24 | hr | | Ki-67 | 1366.10 | p-Rb(S807/S811) | 85.28 | p-cJun(S73) | 140.57 | 2 | WM1552C-signal3 | G6 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 348.86 | p-Rb(S807/S811) | 169.33 | p-ERK(T202/Y204) | 67.27 | 2 | LOXIMVI-signal1 | G6 |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 232.69 | p-Rb(S807/S811) | 95.77 | p-S6(S235/S236 | 414.55 | 2 | WM1552C-signal2 | G6 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 743.36 | p-Rb(S807/S811) | 68.92 | p-ERK(T202/Y204) | 51.05 | 2 | Colo858-signal1 | G6 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.1280 | uM | 24 | hr | | Ki-67 | 2465.90 | p-Rb(S807/S811) | 148.93 | p-cJun(S73) | 37.40 | 2 | LOXIMVI-signal3 | G6 |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 183.28 | p-Rb(S807/S811) | 55.71 | p-S6(S235/S236 | 111.37 | 2 | WM115-signal2 | G6 |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 245.83 | p-Rb(S807/S811) | 61.24 | p-ERK(T202/Y204) | 166.19 | 2 | WM115-signal1 | G6 |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 284.31 | p-Rb(S807/S811) | 88.80 | p-ERK(T202/Y204) | 123.78 | 2 | WM1552C-signal1 | G6 |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 235.54 | p-Rb(S807/S811) | 72.02 | p-ERK(T202/Y204) | 366.04 | 2 | WM115-signal1 | G7 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 511.00 | p-Rb(S807/S811) | 125.23 | p-ERK(T202/Y204) | 254.95 | 2 | Colo858-signal1 | G7 |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.0512 | uM | 24 | hr | | Ki-67 | 764.21 | p-Rb(S807/S811) | 71.74 | p-cJun(S73) | 39.60 | 2 | WM115-signal3 | G7 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 239.08 | p-Rb(S807/S811) | 140.42 | p-S6(S235/S236 | 380.85 | 2 | Colo858-signal2 | G7 |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 191.70 | p-Rb(S807/S811) | 97.70 | p-S6(S235/S236 | 403.09 | 2 | WM1552C-signal2 | G7 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0512 | uM | 24 | hr | | Ki-67 | 2517.90 | p-Rb(S807/S811) | 172.29 | p-cJun(S73) | 48.52 | 2 | LOXIMVI-signal3 | G7 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 325.15 | p-Rb(S807/S811) | 174.20 | p-ERK(T202/Y204) | 85.62 | 2 | LOXIMVI-signal1 | G7 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 230.14 | p-Rb(S807/S811) | 187.63 | p-S6(S235/S236 | 409.48 | 2 | LOXIMVI-signal2 | G7 |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p-Histone H3(S10) | 179.49 | p-Rb(S807/S811) | 67.38 | p-S6(S235/S236 | 142.55 | 2 | WM115-signal2 | G7 |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0512 | uM | 24 | hr | | Ki-67 | 1383.60 | p-Rb(S807/S811) | 94.09 | p-cJun(S73) | 155.54 | 2 | WM1552C-signal3 | G7 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0512 | uM | 24 | hr | | Ki-67 | 1608.40 | p-Rb(S807/S811) | 136.81 | p-cJun(S73) | 31.60 | 2 | Colo858-signal3 | G7 |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 273.71 | p-Rb(S807/S811) | 96.01 | p-ERK(T202/Y204) | 160.08 | 2 | WM1552C-signal1 | G7 |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 202.11 | p-Rb(S807/S811) | 70.44 | p-S6(S235/S236 | 160.70 | 2 | WM115-signal2 | G8 |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 458.83 | p-Rb(S807/S811) | 152.02 | p-ERK(T202/Y204) | 459.90 | 2 | Colo858-signal1 | G8 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p27 Kip1 | 317.63 | p-Rb(S807/S811) | 191.25 | p-ERK(T202/Y204) | 128.24 | 2 | LOXIMVI-signal1 | G8 |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | 258.76 | p-Rb(S807/S811) | 210.04 | p-S6(S235/S236 | 429.29 | 2 | LOXIMVI-signal2 | G8 |