Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| JNK-IN-8 | WM-115 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 243.86 | p-Rb(S807/S811) | 88.86 | p-ERK(T202/Y204) | 663.72 | 1 | WM115-signal1 | D4 | |
| JNK-IN-8 | WM-115 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 237.86 | p-Rb(S807/S811) | 81.40 | p-ERK(T202/Y204) | 668.60 | 2 | WM115-signal1 | E7 | |
| JNK-IN-8 | WM-115 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 226.04 | p-Rb(S807/S811) | 77.90 | p-ERK(T202/Y204) | 670.95 | 2 | WM115-signal1 | E9 | |
| JNK-IN-8 | COLO 858 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 445.16 | p-Rb(S807/S811) | 182.28 | p-ERK(T202/Y204) | 673.10 | 2 | Colo858-signal1 | E5 | |
| JNK-IN-8 | COLO 858 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 430.28 | p-Rb(S807/S811) | 171.77 | p-ERK(T202/Y204) | 673.40 | 1 | Colo858-signal1 | D7 | |
| JNK-IN-8 | COLO 858 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 429.89 | p-Rb(S807/S811) | 170.46 | p-ERK(T202/Y204) | 675.31 | 2 | Colo858-signal1 | E6 | |
| JNK-IN-8 | COLO 858 | 0.3200 | uM | 24 | hr | | p27 Kip1 | 453.72 | p-Rb(S807/S811) | 173.99 | p-ERK(T202/Y204) | 686.67 | 1 | Colo858-signal1 | D5 | |
| JNK-IN-8 | COLO 858 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 450.18 | p-Rb(S807/S811) | 173.59 | p-ERK(T202/Y204) | 691.16 | 1 | Colo858-signal1 | D6 | |
| JNK-IN-8 | COLO 858 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 488.74 | p-Rb(S807/S811) | 190.14 | p-ERK(T202/Y204) | 694.83 | 1 | Colo858-signal1 | D4 | |
| JNK-IN-8 | COLO 858 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 596.02 | p-Rb(S807/S811) | 233.59 | p-ERK(T202/Y204) | 707.32 | 1 | Colo858-signal1 | D3 | |
| JNK-IN-8 | WM-115 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 250.02 | p-Rb(S807/S811) | 88.53 | p-ERK(T202/Y204) | 714.03 | 2 | WM115-signal1 | E3 | |
| JNK-IN-8 | COLO 858 | 0.8000 | uM | 24 | hr | | p27 Kip1 | 504.81 | p-Rb(S807/S811) | 190.17 | p-ERK(T202/Y204) | 719.29 | 2 | Colo858-signal1 | E4 | |
| JNK-IN-8 | WM-115 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 254.69 | p-Rb(S807/S811) | 87.41 | p-ERK(T202/Y204) | 724.06 | 1 | WM115-signal1 | D3 | |
| JNK-IN-8 | COLO 858 | 2.0000 | uM | 24 | hr | | p27 Kip1 | 594.04 | p-Rb(S807/S811) | 231.49 | p-ERK(T202/Y204) | 724.41 | 2 | Colo858-signal1 | E3 | |
| JNK-IN-8 | COLO 858 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 738.89 | p-Rb(S807/S811) | 249.29 | p-ERK(T202/Y204) | 766.04 | 1 | Colo858-signal1 | D2 | |
| JNK-IN-8 | WM-115 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 267.33 | p-Rb(S807/S811) | 79.84 | p-ERK(T202/Y204) | 786.46 | 2 | WM115-signal1 | E2 | |
| JNK-IN-8 | COLO 858 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 839.15 | p-Rb(S807/S811) | 256.43 | p-ERK(T202/Y204) | 789.95 | 2 | Colo858-signal1 | E2 | |
| JNK-IN-8 | WM-115 | 5.0000 | uM | 24 | hr | | p27 Kip1 | 230.19 | p-Rb(S807/S811) | 77.30 | p-ERK(T202/Y204) | 802.24 | 1 | WM115-signal1 | D2 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.0082 | uM | 24 | hr | | p-Histone H3(S10) | p-Rb(S807/S811) | p-S6(S235/S236 | 1 | WM115-signal2 | F9 | |||
| Vemurafenib | WM-115 | JNK-IN-8 | 0.0205 | uM | 24 | hr | | p-Histone H3(S10) | p-Rb(S807/S811) | p-S6(S235/S236 | 1 | WM115-signal2 | F8 |