Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| JNK-IN-8 | LOXIMVI | 0.0000 | uM | 24 | hr | | p27 Kip1 | 351.82 | p-Rb(S807/S811) | 222.97 | p-ERK(T202/Y204) | 225.84 | 2 | LOXIMVI-signal1 | E11 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 362.35 | p-Rb(S807/S811) | 212.69 | p-ERK(T202/Y204) | 226.97 | 1 | LOXIMVI-signal1 | F11 |
| Vemurafenib | WM1552C | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 184.35 | p-Rb(S807/S811) | 50.13 | p-S6(S235/S236 | 228.77 | 2 | WM1552C-signal2 | C2 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 161.46 | p-Rb(S807/S811) | 50.96 | p-S6(S235/S236 | 229.99 | 1 | WM1552C-signal2 | F3 |
| JNK-IN-8 | LOXIMVI | 0.0000 | uM | 24 | hr | | p27 Kip1 | 348.21 | p-Rb(S807/S811) | 217.92 | p-ERK(T202/Y204) | 232.50 | 1 | LOXIMVI-signal1 | D11 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0033 | uM | 24 | hr | | p27 Kip1 | 231.78 | p-Rb(S807/S811) | 102.69 | p-ERK(T202/Y204) | 234.80 | 1 | WM1552C-signal1 | F10 |
| JNK-IN-8 | LOXIMVI | 2.0000 | uM | 24 | hr | | p27 Kip1 | 376.92 | p-Rb(S807/S811) | 258.15 | p-ERK(T202/Y204) | 235.58 | 1 | LOXIMVI-signal1 | D3 | |
| Vemurafenib | WM1552C | 0.0082 | uM | 24 | hr | | p27 Kip1 | 242.69 | p-Rb(S807/S811) | 101.68 | p-ERK(T202/Y204) | 236.72 | 1 | WM1552C-signal1 | B9 | |
| JNK-IN-8 | LOXIMVI | 2.0000 | uM | 24 | hr | | p27 Kip1 | 368.98 | p-Rb(S807/S811) | 251.60 | p-ERK(T202/Y204) | 236.95 | 2 | LOXIMVI-signal1 | E3 | |
| Vemurafenib | WM1552C | 0.0033 | uM | 24 | hr | | p27 Kip1 | 240.73 | p-Rb(S807/S811) | 101.89 | p-ERK(T202/Y204) | 242.12 | 2 | WM1552C-signal1 | C10 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0033 | uM | 24 | hr | | p27 Kip1 | 249.58 | p-Rb(S807/S811) | 104.56 | p-ERK(T202/Y204) | 242.42 | 2 | WM1552C-signal1 | G10 |
| Vemurafenib | WM1552C | 0.0033 | uM | 24 | hr | | p27 Kip1 | 246.89 | p-Rb(S807/S811) | 106.93 | p-ERK(T202/Y204) | 243.94 | 1 | WM1552C-signal1 | B10 | |
| Vemurafenib | WM1552C | 0.0082 | uM | 24 | hr | | p27 Kip1 | 241.99 | p-Rb(S807/S811) | 106.90 | p-ERK(T202/Y204) | 245.11 | 2 | WM1552C-signal1 | C9 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 241.65 | p-Rb(S807/S811) | 104.81 | p-ERK(T202/Y204) | 245.66 | 2 | WM1552C-signal1 | G9 |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 256.65 | p-Rb(S807/S811) | 104.44 | p-ERK(T202/Y204) | 246.25 | 1 | WM1552C-signal1 | F9 |
| JNK-IN-8 | WM1552C | 0.0000 | uM | 24 | hr | | p27 Kip1 | 241.45 | p-Rb(S807/S811) | 105.78 | p-ERK(T202/Y204) | 247.44 | 2 | WM1552C-signal1 | E11 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 146.05 | p-Rb(S807/S811) | 52.87 | p-S6(S235/S236 | 247.44 | 2 | WM1552C-signal2 | G3 |
| JNK-IN-8 | WM1552C | 0.0000 | uM | 24 | hr | | p27 Kip1 | 244.30 | p-Rb(S807/S811) | 104.67 | p-ERK(T202/Y204) | 252.82 | 1 | WM1552C-signal1 | D11 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 239.37 | p-Rb(S807/S811) | 107.35 | p-ERK(T202/Y204) | 253.29 | 1 | WM1552C-signal1 | F11 |
| Vemurafenib | WM1552C | 0.0000 | uM | 24 | hr | | p27 Kip1 | 243.77 | p-Rb(S807/S811) | 107.70 | p-ERK(T202/Y204) | 254.74 | 1 | WM1552C-signal1 | B11 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 511.00 | p-Rb(S807/S811) | 125.23 | p-ERK(T202/Y204) | 254.95 | 2 | Colo858-signal1 | G7 |
| JNK-IN-8 | WM1552C | 0.0033 | uM | 24 | hr | | p27 Kip1 | 241.17 | p-Rb(S807/S811) | 107.27 | p-ERK(T202/Y204) | 256.25 | 2 | WM1552C-signal1 | E10 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 251.50 | p-Rb(S807/S811) | 108.70 | p-ERK(T202/Y204) | 256.82 | 2 | WM1552C-signal1 | G11 |
| JNK-IN-8 | WM1552C | 0.0082 | uM | 24 | hr | | p27 Kip1 | 239.79 | p-Rb(S807/S811) | 104.64 | p-ERK(T202/Y204) | 258.81 | 1 | WM1552C-signal1 | D9 | |
| Vemurafenib | WM1552C | 0.0000 | uM | 24 | hr | | p27 Kip1 | 244.71 | p-Rb(S807/S811) | 105.59 | p-ERK(T202/Y204) | 259.12 | 2 | WM1552C-signal1 | C11 |