Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | WM-115 | 0.3200 | uM | 24 | hr | | Ki-67 | 587.55 | p-Rb(S807/S811) | 26.27 | p-cJun(S73) | 71.93 | 2 | WM115-signal3 | C5 | |
| Vemurafenib | COLO 858 | 0.3200 | uM | 24 | hr | | Ki-67 | 708.08 | p-Rb(S807/S811) | 48.09 | p-cJun(S73) | 72.08 | 1 | Colo858-signal3 | B5 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 173.97 | p-Rb(S807/S811) | 36.66 | p-S6(S235/S236 | 72.75 | 2 | WM115-signal2 | G5 |
| Vemurafenib | COLO 858 | 0.8000 | uM | 24 | hr | | Ki-67 | 678.24 | p-Rb(S807/S811) | 45.98 | p-cJun(S73) | 72.90 | 1 | Colo858-signal3 | B4 | |
| JNK-IN-8 | LOXIMVI | 0.0512 | uM | 24 | hr | | Ki-67 | 2947.60 | p-Rb(S807/S811) | 217.27 | p-cJun(S73) | 73.06 | 1 | LOXIMVI-signal3 | D7 | |
| Vemurafenib | COLO 858 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 849.44 | p-Rb(S807/S811) | 79.57 | p-ERK(T202/Y204) | 75.64 | 1 | Colo858-signal1 | B6 | |
| Vemurafenib | LOXIMVI | 0.3200 | uM | 24 | hr | | Ki-67 | 2470.00 | p-Rb(S807/S811) | 138.22 | p-cJun(S73) | 75.91 | 1 | LOXIMVI-signal3 | B5 | |
| Vemurafenib | LOXIMVI | 0.1280 | uM | 24 | hr | | p27 Kip1 | 364.85 | p-Rb(S807/S811) | 178.03 | p-ERK(T202/Y204) | 76.08 | 2 | LOXIMVI-signal1 | C6 | |
| Vemurafenib | COLO 858 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 144.45 | p-Rb(S807/S811) | 54.34 | p-S6(S235/S236 | 77.03 | 1 | Colo858-signal2 | B5 | |
| Vemurafenib | LOXIMVI | 5.0000 | uM | 24 | hr | | Ki-67 | 1979.00 | p-Rb(S807/S811) | 73.51 | p-cJun(S73) | 77.14 | 2 | LOXIMVI-signal3 | C2 | |
| Vemurafenib | WM-115 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 180.39 | p-Rb(S807/S811) | 38.47 | p-S6(S235/S236 | 77.58 | 1 | WM115-signal2 | B5 | |
| Vemurafenib | WM-115 | JNK-IN-8 | 0.3200 | uM | 24 | hr | | p-Histone H3(S10) | 206.61 | p-Rb(S807/S811) | 41.17 | p-S6(S235/S236 | 77.73 | 1 | WM115-signal2 | F5 |
| Vemurafenib | WM-115 | 0.3200 | uM | 24 | hr | | Ki-67 | 721.37 | p-Rb(S807/S811) | 38.52 | p-cJun(S73) | 78.48 | 1 | WM115-signal3 | B5 | |
| Vemurafenib | WM-115 | 0.8000 | uM | 24 | hr | | Ki-67 | 663.63 | p-Rb(S807/S811) | 39.63 | p-cJun(S73) | 79.94 | 1 | WM115-signal3 | B4 | |
| Vemurafenib | LOXIMVI | 5.0000 | uM | 24 | hr | | Ki-67 | 2047.60 | p-Rb(S807/S811) | 78.14 | p-cJun(S73) | 80.02 | 1 | LOXIMVI-signal3 | B2 | |
| Vemurafenib | COLO 858 | JNK-IN-8 | 0.1280 | uM | 24 | hr | | p27 Kip1 | 738.58 | p-Rb(S807/S811) | 81.58 | p-ERK(T202/Y204) | 80.67 | 1 | Colo858-signal1 | F6 |
| Vemurafenib | LOXIMVI | 0.8000 | uM | 24 | hr | | Ki-67 | 2448.30 | p-Rb(S807/S811) | 126.79 | p-cJun(S73) | 81.56 | 2 | LOXIMVI-signal3 | C4 | |
| Vemurafenib | LOXIMVI | 0.8000 | uM | 24 | hr | | Ki-67 | 2357.40 | p-Rb(S807/S811) | 128.12 | p-cJun(S73) | 82.54 | 1 | LOXIMVI-signal3 | B4 | |
| Vemurafenib | WM-115 | 0.8000 | uM | 24 | hr | | Ki-67 | 666.78 | p-Rb(S807/S811) | 38.55 | p-cJun(S73) | 82.82 | 2 | WM115-signal3 | C4 | |
| JNK-IN-8 | LOXIMVI | 0.0205 | uM | 24 | hr | | Ki-67 | 2981.20 | p-Rb(S807/S811) | 212.09 | p-cJun(S73) | 82.93 | 2 | LOXIMVI-signal3 | E8 | |
| Vemurafenib | LOXIMVI | 0.3200 | uM | 24 | hr | | Ki-67 | 2387.10 | p-Rb(S807/S811) | 134.09 | p-cJun(S73) | 83.10 | 2 | LOXIMVI-signal3 | C5 | |
| Vemurafenib | LOXIMVI | 0.1280 | uM | 24 | hr | | p27 Kip1 | 366.51 | p-Rb(S807/S811) | 180.61 | p-ERK(T202/Y204) | 84.29 | 1 | LOXIMVI-signal1 | B6 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0082 | uM | 24 | hr | | Ki-67 | 2667.60 | p-Rb(S807/S811) | 189.57 | p-cJun(S73) | 84.40 | 2 | LOXIMVI-signal3 | G9 |
| Vemurafenib | LOXIMVI | 2.0000 | uM | 24 | hr | | Ki-67 | 2153.30 | p-Rb(S807/S811) | 92.72 | p-cJun(S73) | 84.91 | 2 | LOXIMVI-signal3 | C3 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0512 | uM | 24 | hr | | p27 Kip1 | 325.15 | p-Rb(S807/S811) | 174.20 | p-ERK(T202/Y204) | 85.62 | 2 | LOXIMVI-signal1 | G7 |