Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| JNK-IN-8 | WM1552C | 0.0000 | uM | 24 | hr | | p27 Kip1 | 244.30 | p-Rb(S807/S811) | 104.67 | p-ERK(T202/Y204) | 252.82 | 1 | WM1552C-signal1 | D11 | |
| JNK-IN-8 | WM1552C | 0.0000 | uM | 24 | hr | | p27 Kip1 | 241.45 | p-Rb(S807/S811) | 105.78 | p-ERK(T202/Y204) | 247.44 | 2 | WM1552C-signal1 | E11 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 146.05 | p-Rb(S807/S811) | 52.87 | p-S6(S235/S236 | 247.44 | 2 | WM1552C-signal2 | G3 |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 256.65 | p-Rb(S807/S811) | 104.44 | p-ERK(T202/Y204) | 246.25 | 1 | WM1552C-signal1 | F9 |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0082 | uM | 24 | hr | | p27 Kip1 | 241.65 | p-Rb(S807/S811) | 104.81 | p-ERK(T202/Y204) | 245.66 | 2 | WM1552C-signal1 | G9 |
| Vemurafenib | WM1552C | 0.0082 | uM | 24 | hr | | p27 Kip1 | 241.99 | p-Rb(S807/S811) | 106.90 | p-ERK(T202/Y204) | 245.11 | 2 | WM1552C-signal1 | C9 | |
| Vemurafenib | WM1552C | 0.0033 | uM | 24 | hr | | p27 Kip1 | 246.89 | p-Rb(S807/S811) | 106.93 | p-ERK(T202/Y204) | 243.94 | 1 | WM1552C-signal1 | B10 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0033 | uM | 24 | hr | | p27 Kip1 | 249.58 | p-Rb(S807/S811) | 104.56 | p-ERK(T202/Y204) | 242.42 | 2 | WM1552C-signal1 | G10 |
| Vemurafenib | WM1552C | 0.0033 | uM | 24 | hr | | p27 Kip1 | 240.73 | p-Rb(S807/S811) | 101.89 | p-ERK(T202/Y204) | 242.12 | 2 | WM1552C-signal1 | C10 | |
| JNK-IN-8 | LOXIMVI | 2.0000 | uM | 24 | hr | | p27 Kip1 | 368.98 | p-Rb(S807/S811) | 251.60 | p-ERK(T202/Y204) | 236.95 | 2 | LOXIMVI-signal1 | E3 | |
| Vemurafenib | WM1552C | 0.0082 | uM | 24 | hr | | p27 Kip1 | 242.69 | p-Rb(S807/S811) | 101.68 | p-ERK(T202/Y204) | 236.72 | 1 | WM1552C-signal1 | B9 | |
| JNK-IN-8 | LOXIMVI | 2.0000 | uM | 24 | hr | | p27 Kip1 | 376.92 | p-Rb(S807/S811) | 258.15 | p-ERK(T202/Y204) | 235.58 | 1 | LOXIMVI-signal1 | D3 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 0.0033 | uM | 24 | hr | | p27 Kip1 | 231.78 | p-Rb(S807/S811) | 102.69 | p-ERK(T202/Y204) | 234.80 | 1 | WM1552C-signal1 | F10 |
| JNK-IN-8 | LOXIMVI | 0.0000 | uM | 24 | hr | | p27 Kip1 | 348.21 | p-Rb(S807/S811) | 217.92 | p-ERK(T202/Y204) | 232.50 | 1 | LOXIMVI-signal1 | D11 | |
| Vemurafenib | WM1552C | JNK-IN-8 | 2.0000 | uM | 24 | hr | | p-Histone H3(S10) | 161.46 | p-Rb(S807/S811) | 50.96 | p-S6(S235/S236 | 229.99 | 1 | WM1552C-signal2 | F3 |
| Vemurafenib | WM1552C | 5.0000 | uM | 24 | hr | | p-Histone H3(S10) | 184.35 | p-Rb(S807/S811) | 50.13 | p-S6(S235/S236 | 228.77 | 2 | WM1552C-signal2 | C2 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 362.35 | p-Rb(S807/S811) | 212.69 | p-ERK(T202/Y204) | 226.97 | 1 | LOXIMVI-signal1 | F11 |
| JNK-IN-8 | LOXIMVI | 0.0000 | uM | 24 | hr | | p27 Kip1 | 351.82 | p-Rb(S807/S811) | 222.97 | p-ERK(T202/Y204) | 225.84 | 2 | LOXIMVI-signal1 | E11 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.8000 | uM | 24 | hr | | p-Histone H3(S10) | 229.22 | p-Rb(S807/S811) | 100.85 | p-S6(S235/S236 | 225.60 | 1 | LOXIMVI-signal2 | F4 |
| JNK-IN-8 | LOXIMVI | 0.8000 | uM | 24 | hr | | p27 Kip1 | 336.96 | p-Rb(S807/S811) | 231.40 | p-ERK(T202/Y204) | 222.11 | 1 | LOXIMVI-signal1 | D4 | |
| JNK-IN-8 | LOXIMVI | 0.1280 | uM | 24 | hr | | p27 Kip1 | 319.20 | p-Rb(S807/S811) | 216.66 | p-ERK(T202/Y204) | 220.23 | 1 | LOXIMVI-signal1 | D6 | |
| Vemurafenib | COLO 858 | 0.1280 | uM | 24 | hr | | p-Histone H3(S10) | 204.59 | p-Rb(S807/S811) | 83.78 | p-S6(S235/S236 | 220.01 | 1 | Colo858-signal2 | B6 | |
| JNK-IN-8 | LOXIMVI | 0.3200 | uM | 24 | hr | | p27 Kip1 | 312.28 | p-Rb(S807/S811) | 224.98 | p-ERK(T202/Y204) | 217.75 | 1 | LOXIMVI-signal1 | D5 | |
| Vemurafenib | LOXIMVI | JNK-IN-8 | 0.0000 | uM | 24 | hr | | p27 Kip1 | 351.99 | p-Rb(S807/S811) | 215.31 | p-ERK(T202/Y204) | 217.61 | 2 | LOXIMVI-signal1 | G11 |
| Vemurafenib | LOXIMVI | 0.0000 | uM | 24 | hr | | p27 Kip1 | 346.49 | p-Rb(S807/S811) | 215.22 | p-ERK(T202/Y204) | 215.11 | 1 | LOXIMVI-signal1 | B11 |