Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by imaging - Dataset (ID:20219)
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| Small Molecule | Cell Line | 2nd Drug | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Image | Measured Protein (Alexa Fluor 488) | Measured Protein Value (488) | Measured Protein (Alexa Fluor 568) | Measured Protein Value (568) | Measured Protein (Alexa Fluor 647) | Measured Protein Value (647) | Replicate | Plate Name | Plate Well |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Vemurafenib | WM1552C | 0.8000 | uM | 24 | hr | | p27 Kip1 | 440.30 | p-Rb(S807/S811) | 48.95 | p-ERK(T202/Y204) | 28.75 | 2 | WM1552C-signal1 | C4 | |
| JNK-IN-8 | WM1552C | 5.0000 | uM | 24 | hr | | p27 Kip1 | 304.56 | p-Rb(S807/S811) | 111.24 | p-ERK(T202/Y204) | 317.45 | 1 | WM1552C-signal1 | D2 | |
| Vemurafenib | WM1552C | 0.1280 | uM | 24 | hr | | p27 Kip1 | 284.96 | p-Rb(S807/S811) | 88.69 | p-ERK(T202/Y204) | 119.29 | 1 | WM1552C-signal1 | B6 | |
| Vemurafenib | WM1552C | 2.0000 | uM | 24 | hr | | p27 Kip1 | 472.02 | p-Rb(S807/S811) | 42.22 | p-ERK(T202/Y204) | 19.71 | 2 | WM1552C-signal1 | C3 | |
| Vemurafenib | WM1552C | 0.0205 | uM | 24 | hr | | p27 Kip1 | 249.99 | p-Rb(S807/S811) | 102.20 | p-ERK(T202/Y204) | 212.75 | 2 | WM1552C-signal1 | C8 | |
| JNK-IN-8 | WM1552C | 0.0000 | uM | 24 | hr | | p27 Kip1 | 244.30 | p-Rb(S807/S811) | 104.67 | p-ERK(T202/Y204) | 252.82 | 1 | WM1552C-signal1 | D11 | |
| Vemurafenib | WM1552C | 5.0000 | uM | 24 | hr | | p27 Kip1 | 446.61 | p-Rb(S807/S811) | 44.76 | p-ERK(T202/Y204) | 19.95 | 1 | WM1552C-signal1 | B2 | |
| Vemurafenib | WM1552C | 0.3200 | uM | 24 | hr | | p27 Kip1 | 337.86 | p-Rb(S807/S811) | 61.82 | p-ERK(T202/Y204) | 50.97 | 1 | WM1552C-signal1 | B5 | |
| Vemurafenib | WM1552C | 0.0082 | uM | 24 | hr | | p27 Kip1 | 242.69 | p-Rb(S807/S811) | 101.68 | p-ERK(T202/Y204) | 236.72 | 1 | WM1552C-signal1 | B9 | |
| Vemurafenib | WM1552C | 5.0000 | uM | 24 | hr | | p27 Kip1 | 433.95 | p-Rb(S807/S811) | 51.26 | p-ERK(T202/Y204) | 22.13 | 2 | WM1552C-signal1 | C2 | |
| Vemurafenib | WM1552C | 0.1280 | uM | 24 | hr | | p27 Kip1 | 271.43 | p-Rb(S807/S811) | 88.61 | p-ERK(T202/Y204) | 136.09 | 2 | WM1552C-signal1 | C6 | |
| Vemurafenib | WM1552C | 0.0512 | uM | 24 | hr | | p27 Kip1 | 262.18 | p-Rb(S807/S811) | 94.16 | p-ERK(T202/Y204) | 164.16 | 2 | WM1552C-signal1 | C7 | |
| Vemurafenib | WM1552C | 0.0082 | uM | 24 | hr | | p27 Kip1 | 241.99 | p-Rb(S807/S811) | 106.90 | p-ERK(T202/Y204) | 245.11 | 2 | WM1552C-signal1 | C9 | |
| JNK-IN-8 | WM1552C | 0.0033 | uM | 24 | hr | | p27 Kip1 | 237.48 | p-Rb(S807/S811) | 106.12 | p-ERK(T202/Y204) | 259.42 | 1 | WM1552C-signal1 | D10 | |
| Vemurafenib | WM1552C | 0.0033 | uM | 24 | hr | | p27 Kip1 | 246.89 | p-Rb(S807/S811) | 106.93 | p-ERK(T202/Y204) | 243.94 | 1 | WM1552C-signal1 | B10 | |
| Vemurafenib | WM1552C | 0.0000 | uM | 24 | hr | | p27 Kip1 | 243.77 | p-Rb(S807/S811) | 107.70 | p-ERK(T202/Y204) | 254.74 | 1 | WM1552C-signal1 | B11 | |
| Vemurafenib | WM1552C | 2.0000 | uM | 24 | hr | | p27 Kip1 | 473.39 | p-Rb(S807/S811) | 42.61 | p-ERK(T202/Y204) | 22.62 | 1 | WM1552C-signal1 | B3 | |
| Vemurafenib | WM1552C | 0.8000 | uM | 24 | hr | | p27 Kip1 | 442.40 | p-Rb(S807/S811) | 51.64 | p-ERK(T202/Y204) | 26.95 | 1 | WM1552C-signal1 | B4 | |
| Vemurafenib | WM1552C | 0.0512 | uM | 24 | hr | | p27 Kip1 | 269.92 | p-Rb(S807/S811) | 94.99 | p-ERK(T202/Y204) | 160.06 | 1 | WM1552C-signal1 | B7 | |
| Vemurafenib | WM1552C | 0.0205 | uM | 24 | hr | | p27 Kip1 | 252.92 | p-Rb(S807/S811) | 100.14 | p-ERK(T202/Y204) | 207.93 | 1 | WM1552C-signal1 | B8 | |
| Vemurafenib | WM1552C | 0.0033 | uM | 24 | hr | | p27 Kip1 | 240.73 | p-Rb(S807/S811) | 101.89 | p-ERK(T202/Y204) | 242.12 | 2 | WM1552C-signal1 | C10 | |
| Vemurafenib | WM1552C | 0.0000 | uM | 24 | hr | | p27 Kip1 | 244.71 | p-Rb(S807/S811) | 105.59 | p-ERK(T202/Y204) | 259.12 | 2 | WM1552C-signal1 | C11 | |
| Vemurafenib | WM1552C | 0.3200 | uM | 24 | hr | | p27 Kip1 | 349.70 | p-Rb(S807/S811) | 71.79 | p-ERK(T202/Y204) | 64.17 | 2 | WM1552C-signal1 | C5 | |
| JNK-IN-8 | WM1552C | 0.8000 | uM | 24 | hr | | p27 Kip1 | 247.69 | p-Rb(S807/S811) | 115.02 | p-ERK(T202/Y204) | 276.76 | 1 | WM1552C-signal1 | D4 | |
| JNK-IN-8 | WM1552C | 2.0000 | uM | 24 | hr | | p27 Kip1 | 258.20 | p-Rb(S807/S811) | 113.91 | p-ERK(T202/Y204) | 277.27 | 1 | WM1552C-signal1 | D3 |