Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)

August 25, 2015: The data file for this dataset was updated to include additional DMSO control data that were inadvertently omitted from the original version. The Assay Protocol description of how experimental data were normalized to DMSO control data also was clarified (point 9).

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Small Molecule Cell Line Drug Concentration Conc Unit Time Point Time Point Unit Replicate Scanner/Software pMEK(S217/221) Normalized Median pMEK(S217/221) pERK(T202/Y204) Normalized Median pERK(T202/Y204) p-p90RSK(S380) Normalized Median p-p90RSK(S380) p-p90RSK(T573) Normalized Median p-p90RSK(T573) p-AKT(T308) Normalized Median p-AKT(T308) p-AKT(S473) Normalized Median p-AKT(S473) p-mTOR(S2448) Normalized Median p-mTOR(S2448) p-p70S6K(T421/S424) Normalized Median p-p70S6K(T421/S424) p-p70S6K(T389) Normalized Median p-p70S6K(T389) p-S6(S235/236) Normalized Median p-S6(S235/236) p-AMPK(T172) Normalized Median p-AMPK(T172) p-JNK(T183/Y185) Normalized Median p-JNK(T183/Y185) Total c-Jun Normalized Median Total c-Jun p-cJun(S63) Normalized Median p-cJun(S63) p-P38(T180/Y182) Normalized Median p-P38(T180/Y182) p-HSP27(S82) Normalized Median p-HSP27(S82) p-NFKB(S536) Normalized Median p-NFKB(S536) Bim Normalized Median Bim cPARP Normalized Median cPARP p-Histone H3(S10) Normalized Median p-Histone H3(S10) p27 Kip1 Normalized Median p27 Kip1
COLO 858 0.0000
uM
 10
hr
 5
Odyssey/Microvigene
 1.0905 2.6810 0.1733 0.3003 0.1876 0.3311 0.2070 0.2660 1.2165 0.9804 0.5747 0.2008 0.2421 0.3704 0.5155 0.2045 0.1235 0.5814 0.7194
COLO 858 0.0000
uM
 10
hr
 5
Odyssey/Microvigene
 1.0929 2.6247 0.1689 0.2639 0.1600 0.3497 0.2198 0.3460 1.2361 0.9346 0.5525 0.1919 0.2604 0.4065 0.5525 0.1805 0.1520 0.7194 0.7269
COLO 858 0.0000
uM
 10
hr
 5
Odyssey/Microvigene
 0.9259 2.0964 0.2101 0.1385 0.3003 0.1770 0.2105 1.3423 0.7874 0.4348 0.1300 0.1825 0.3125 0.3623 0.0980 0.1359 0.5618 0.6148
COLO 858 0.0000
uM
 10
hr
 5
Odyssey/Microvigene
 0.7634 1.4771 0.1518 0.2151 0.1348 0.2747 0.1776 0.2320 0.9434 0.6250 0.4167 0.1539 0.1727 0.2994 0.3704 0.1335 0.1065 0.6250 0.4745
COLO 858 0.0000
uM
 10
hr
 5
Odyssey/Microvigene
 1.0627 2.3148 0.1961 0.3003 0.1502 0.3226 0.2101 0.2801 1.1442 0.9901 0.5556 0.1647 0.2101 0.2907 0.4484 0.1684 0.1350 0.6211 0.6465
COLO 858 0.0000
uM
 10
hr
 5
Odyssey/Microvigene
 1.0846 2.4096 0.1692 0.2703 0.1876 0.3704 0.2101 0.3030 1.2771 0.9259 0.5917 0.1838 0.2558 0.3356 0.4975 0.1585 0.1546 0.7634 0.6700
COLO 858 0.0000
uM
 10
hr
 5
Odyssey/Microvigene
 0.9615 2.0790 0.1563 0.2053 0.1795 0.2976 0.1869 0.2353 1.3021 0.7692 0.3831 0.1629 0.1934 0.2985 0.4546 0.0671 0.1133 0.6098 0.6142
COLO 858 0.0000
uM
 10
hr
 5
Odyssey/Microvigene
 0.7407 1.4815 0.1575 0.2294 0.2033 0.2398 0.2016 0.2833 1.0183 0.6061 0.3953 0.1942 0.1980 0.2801 0.4608 0.1004 0.1041 0.6329 0.4958
COLO 858 0.0000
uM
 10
hr
 6
Odyssey/Microvigene
 0.8547 1.6103 0.1175 0.1818 0.1393 0.2247 0.1464 0.1739 1.0081 0.6135 0.3546 0.1294 0.1410 0.2469 0.3165 0.0629 0.0752 0.4386 0.5424
COLO 858 0.0000
uM
 10
hr
 6
Odyssey/Microvigene
COLO 858 0.0000
uM
 10
hr
 6
Odyssey/Microvigene
 0.6579 1.0787 0.1284 0.1692 0.1531 0.1923 0.1605 0.1859 0.6711 0.4608 0.3215 0.1362 0.1468 0.2976 0.3704 0.0433 0.0787 0.4975 0.4048
COLO 858 0.0000
uM
 10
hr
 6
Odyssey/Microvigene
 0.7246 1.2255 0.1359 0.1905 0.1595 0.2387 0.1776 0.2217 0.9091 0.5291 0.3215 0.1350 0.1664 0.3086 0.3953 0.0787 0.0769 0.5747 0.4453
COLO 858 0.0000
uM
 10
hr
 6
Odyssey/Microvigene
COLO 858 0.0000
uM
 10
hr
 6
Odyssey/Microvigene
COLO 858 0.0000
uM
 10
hr
 6
Odyssey/Microvigene
 0.6711 1.1013 0.1284 0.1832 0.1541 0.1848 0.1721 0.2105 0.7092 0.4762 0.3290 0.1407 0.1709 0.2488 0.3663 0.0581 0.0769 0.5128 0.4091
COLO 858 0.0000
uM
 10
hr
 6
Odyssey/Microvigene
 0.7299 1.3661 0.1335 0.1792 0.1563 0.2299 0.1689 0.2151 0.9524 0.5128 0.3268 0.1493 0.1686 0.2506 0.3610 0.0893 0.0787 0.5952 0.4474
COLO 858 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 1.0111 2.2779 0.1311 0.2160 0.1110 0.2899 0.1355 0.1890 1.4903 0.7634 0.4082 0.1346 0.1577 0.2841 0.3356 0.0617 0.1274 0.4951 0.6142
COLO 858 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 0.9524 2.0492 0.1287 0.2016 0.1074 0.3185 0.1458 0.1934 1.4368 0.7692 0.3922 0.1232 0.1742 0.3718 0.0549 0.1174 0.5556 0.6202
COLO 858 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 0.7634 1.6447 0.1131 0.1761 0.1299 0.2398 0.1502 0.1894 0.9615 0.5882 0.3226 0.1196 0.1397 0.2740 0.3155 0.0420 0.0840 0.5376 0.5222
COLO 858 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 0.6944 1.3369 0.1076 0.1984 0.1255 0.2849 0.1597 0.1984 0.9346 0.5435 0.3155 0.1548 0.1466 0.2967 0.3448 0.0719 0.0901 0.6410 0.4651
COLO 858 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 1.0000 2.1786 0.1121 0.2160 0.1220 0.3003 0.1468 0.1934 1.4327 0.8265 0.3906 0.1437 0.1684 0.2359 0.3521 0.0521 0.0926 0.5465 0.6057
COLO 858 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 0.9524 2.0833 0.1131 0.2049 0.1148 0.3086 0.1587 0.1996 0.4255 0.8333 0.3846 0.1395 0.1825 0.2770 0.3546 0.0826 0.1259 0.6061 0.6065
COLO 858 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 0.7813 1.6750 0.1433 0.1912 0.1678 0.2463 0.1733 0.2353 1.1001 0.6061 0.3497 0.1522 0.1712 0.2959 0.3906 0.0637 0.0980 0.5814 0.5231
COLO 858 0.0000
uM
 10
hr
 7
Odyssey/Microvigene
 0.7407 1.4124 0.1520 0.2165 0.1597 0.2778 0.1890 1.1123 0.5650 0.3718 0.1563 0.1942 0.3077 0.4016 0.1083 0.6536 0.5196
COLO 858 0.0000
uM
 10
hr
 8
Odyssey/Microvigene
 0.6711 1.2484 0.1377 0.2160 0.1639 0.2101 0.1678 0.2283 0.8929 0.5291 0.3205 0.1536 0.1709 0.3030 0.3690 0.1004 0.0794 0.5556 0.4428