Phosphorylation state and protein levels measured in BRAF(V600E/D) melanoma cell lines monitored by Reverse Phase Protein Arrays (RPPA) - Dataset (ID:20218)
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Small Molecule | Cell Line | Drug Concentration | Conc Unit | Time Point | Time Point Unit | Replicate | Scanner/Software | pMEK(S217/221) | Normalized Median pMEK(S217/221) | pERK(T202/Y204) | Normalized Median pERK(T202/Y204) | p-p90RSK(S380) | Normalized Median p-p90RSK(S380) | p-p90RSK(T573) | Normalized Median p-p90RSK(T573) | p-AKT(T308) | Normalized Median p-AKT(T308) | p-AKT(S473) | Normalized Median p-AKT(S473) | p-mTOR(S2448) | Normalized Median p-mTOR(S2448) | p-p70S6K(T421/S424) | Normalized Median p-p70S6K(T421/S424) | p-p70S6K(T389) | Normalized Median p-p70S6K(T389) | p-S6(S235/236) | Normalized Median p-S6(S235/236) | p-AMPK(T172) | Normalized Median p-AMPK(T172) | p-JNK(T183/Y185) | Normalized Median p-JNK(T183/Y185) | Total c-Jun | Normalized Median Total c-Jun | p-cJun(S63) | Normalized Median p-cJun(S63) | p-P38(T180/Y182) | Normalized Median p-P38(T180/Y182) | p-HSP27(S82) | Normalized Median p-HSP27(S82) | p-NFKB(S536) | Normalized Median p-NFKB(S536) | Bim | Normalized Median Bim | cPARP | Normalized Median cPARP | p-Histone H3(S10) | Normalized Median p-Histone H3(S10) | p27 Kip1 | Normalized Median p27 Kip1 |
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PLX-4720 | COLO 858 | 0.3160 | uM | 48 | hr | 4 | InnoScan/Mapix | 0.0092 | -1.8299 | 0.1905 | -1.5013 | 0.0040 | 0.0578 | 0.0027 | 0.1172 | 0.0132 | 1.3332 | 0.0199 | 1.3304 | 0.0079 | 0.4941 | 0.0086 | 0.0639 | -0.2919 | 0.0919 | -2.0231 | 0.0308 | -0.7849 | 0.0037 | 0.8010 | 0.0425 | 1.3233 | 0.0157 | 1.1971 | 0.0441 | 0.5909 | 0.0044 | 0.0347 | 0.0818 | 0.5369 | 0.0179 | 1.3219 | 0.0235 | 1.5529 | 0.0464 | -0.9815 | 0.0369 | 1.6887 | |
PLX-4720 | COLO 858 | 0.3160 | uM | 48 | hr | 5 | InnoScan/Mapix | 0.0129 | -1.8299 | 0.1458 | -1.5013 | 0.0044 | 0.0578 | 0.0035 | 0.1172 | 0.0225 | 1.3332 | 0.0194 | 1.3304 | 0.0116 | 0.4941 | 0.0151 | 0.0639 | 0.0496 | -0.2919 | 0.0797 | -2.0231 | 0.0452 | -0.7849 | 0.0048 | 0.8010 | 0.0379 | 1.3233 | 0.0174 | 1.1971 | 0.0300 | 0.5909 | 0.0068 | 0.0347 | 0.0671 | 0.5369 | 0.0485 | 1.3219 | 0.0178 | 1.5529 | 0.0343 | -0.9815 | 0.0479 | 1.6887 |
PLX-4720 | COLO 858 | 0.3160 | uM | 48 | hr | 6 | InnoScan/Mapix | 0.0143 | -1.8299 | 0.2342 | -1.5013 | 0.0045 | 0.0578 | 0.0039 | 0.1172 | 0.0208 | 1.3332 | 0.0239 | 1.3304 | 0.0147 | 0.4941 | 0.0180 | 0.0639 | 0.0591 | -0.2919 | 0.1014 | -2.0231 | 0.0446 | -0.7849 | 0.0057 | 0.8010 | 0.0427 | 1.3233 | 0.0153 | 1.1971 | 0.0413 | 0.5909 | 0.0065 | 0.0347 | 0.0920 | 0.5369 | 0.0338 | 1.3219 | 0.0155 | 1.5529 | 0.0467 | -0.9815 | 0.0615 | 1.6887 |
PLX-4720 | COLO 858 | 0.3160 | uM | 48 | hr | 7 | InnoScan/Mapix | 0.0139 | -1.8299 | 0.1707 | -1.5013 | 0.0041 | 0.0578 | 0.0028 | 0.1172 | 0.0142 | 1.3332 | 0.0132 | 1.3304 | 0.0121 | 0.4941 | 0.0132 | 0.0639 | 0.0530 | -0.2919 | 0.0932 | -2.0231 | 0.0384 | -0.7849 | 0.0054 | 0.8010 | 0.0421 | 1.3233 | 0.0189 | 1.1971 | 0.0289 | 0.5909 | 0.0041 | 0.0347 | 0.0916 | 0.5369 | 0.0236 | 1.3219 | 0.0246 | 1.5529 | 0.0431 | -0.9815 | 0.0517 | 1.6887 |
PLX-4720 | COLO 858 | 0.3160 | uM | 48 | hr | 8 | InnoScan/Mapix | 0.0157 | -1.8299 | 0.3068 | -1.5013 | 0.0578 | 0.0054 | 0.1172 | 0.0160 | 1.3332 | 0.0167 | 1.3304 | 0.0158 | 0.4941 | 0.0198 | 0.0639 | 0.0596 | -0.2919 | 0.1060 | -2.0231 | 0.0387 | -0.7849 | 0.0076 | 0.8010 | 0.0423 | 1.3233 | 0.0193 | 1.1971 | 0.0442 | 0.5909 | 0.0053 | 0.0347 | 0.1005 | 0.5369 | 0.0186 | 1.3219 | 0.0221 | 1.5529 | 0.0522 | -0.9815 | 0.0661 | 1.6887 | |
PLX-4720 | COLO 858 | 1.0000 | uM | 1 | hr | 1 | InnoScan/Mapix | 0.0068 | -2.9026 | 0.0244 | -4.3976 | 0.0044 | -0.5066 | -0.1735 | 0.0127 | 0.7476 | 0.0128 | 0.7889 | 0.0082 | -0.2825 | 0.0134 | -0.6528 | 0.0560 | -0.4990 | 0.3279 | -0.1921 | 0.0475 | -0.5076 | -0.0302 | 0.0130 | -0.3580 | 0.0081 | -0.0780 | 0.0086 | -1.2930 | 0.0054 | -0.1056 | 0.0653 | 0.1698 | 0.0189 | 0.6075 | 0.8786 | 0.1013 | 0.4159 | 0.0180 | -0.3293 | |||
PLX-4720 | COLO 858 | 1.0000 | uM | 1 | hr | 2 | InnoScan/Mapix | 0.0069 | -2.9026 | 0.0290 | -4.3976 | 0.0028 | -0.5066 | 0.0030 | -0.1735 | 0.0131 | 0.7476 | 0.0156 | 0.7889 | 0.0073 | -0.2825 | 0.0104 | -0.6528 | 0.0602 | -0.4990 | 0.4016 | -0.1921 | 0.0351 | -0.5076 | 0.0031 | -0.0302 | 0.0150 | -0.3580 | 0.0072 | -0.0780 | 0.0088 | -1.2930 | 0.0052 | -0.1056 | 0.1698 | 0.0133 | 0.6075 | 0.0156 | 0.8786 | 0.1812 | 0.4159 | 0.0134 | -0.3293 | |
PLX-4720 | COLO 858 | 1.0000 | uM | 1 | hr | 3 | InnoScan/Mapix | 0.0076 | -2.9026 | 0.0291 | -4.3976 | 0.0031 | -0.5066 | 0.0039 | -0.1735 | 0.0151 | 0.7476 | 0.0137 | 0.7889 | 0.0074 | -0.2825 | 0.0113 | -0.6528 | 0.0377 | -0.4990 | 0.3425 | -0.1921 | 0.0418 | -0.5076 | 0.0031 | -0.0302 | 0.0169 | -0.3580 | 0.0084 | -0.0780 | 0.0105 | -1.2930 | 0.0062 | -0.1056 | 0.0624 | 0.1698 | 0.0214 | 0.6075 | 0.0124 | 0.8786 | 0.1063 | 0.4159 | 0.0172 | -0.3293 |
PLX-4720 | COLO 858 | 1.0000 | uM | 1 | hr | 4 | InnoScan/Mapix | 0.0070 | -2.9026 | 0.0234 | -4.3976 | 0.0027 | -0.5066 | 0.0030 | -0.1735 | 0.0153 | 0.7476 | 0.7889 | 0.0078 | -0.2825 | 0.0109 | -0.6528 | 0.0437 | -0.4990 | 0.3106 | -0.1921 | 0.0405 | -0.5076 | 0.0033 | -0.0302 | 0.0170 | -0.3580 | 0.0077 | -0.0780 | 0.0113 | -1.2930 | 0.0047 | -0.1056 | 0.0684 | 0.1698 | 0.0210 | 0.6075 | 0.0123 | 0.8786 | 0.1302 | 0.4159 | 0.0144 | -0.3293 | |
PLX-4720 | COLO 858 | 1.0000 | uM | 1 | hr | 5 | InnoScan/Mapix | 0.0058 | -2.9026 | 0.0181 | -4.3976 | 0.0030 | -0.5066 | 0.0037 | -0.1735 | 0.0109 | 0.7476 | 0.0127 | 0.7889 | 0.0067 | -0.2825 | 0.0092 | -0.6528 | 0.0470 | -0.4990 | 0.2755 | -0.1921 | 0.0490 | -0.5076 | 0.0032 | -0.0302 | 0.0119 | -0.3580 | -0.0780 | 0.0097 | -1.2930 | 0.0072 | -0.1056 | 0.1698 | 0.0142 | 0.6075 | 0.0125 | 0.8786 | 0.0830 | 0.4159 | 0.0097 | -0.3293 | ||
PLX-4720 | COLO 858 | 1.0000 | uM | 1 | hr | 6 | InnoScan/Mapix | 0.0054 | -2.9026 | 0.0170 | -4.3976 | 0.0047 | -0.5066 | 0.0026 | -0.1735 | 0.0098 | 0.7476 | 0.0141 | 0.7889 | 0.0062 | -0.2825 | 0.0077 | -0.6528 | 0.0646 | -0.4990 | 0.2463 | -0.1921 | 0.0438 | -0.5076 | 0.0026 | -0.0302 | 0.0126 | -0.3580 | 0.0066 | -0.0780 | 0.0084 | -1.2930 | 0.0060 | -0.1056 | 0.0679 | 0.1698 | 0.0123 | 0.6075 | 0.0107 | 0.8786 | 0.1206 | 0.4159 | 0.0084 | -0.3293 |
PLX-4720 | COLO 858 | 1.0000 | uM | 1 | hr | 7 | InnoScan/Mapix | 0.0062 | -2.9026 | 0.0195 | -4.3976 | 0.0026 | -0.5066 | 0.0030 | -0.1735 | 0.0104 | 0.7476 | 0.0121 | 0.7889 | 0.0066 | -0.2825 | 0.0086 | -0.6528 | 0.0370 | -0.4990 | 0.3497 | -0.1921 | 0.0492 | -0.5076 | 0.0027 | -0.0302 | 0.0124 | -0.3580 | 0.0064 | -0.0780 | 0.0089 | -1.2930 | 0.0067 | -0.1056 | 0.0664 | 0.1698 | 0.0154 | 0.6075 | 0.0106 | 0.8786 | 0.1215 | 0.4159 | 0.0136 | -0.3293 |
PLX-4720 | COLO 858 | 1.0000 | uM | 1 | hr | 8 | InnoScan/Mapix | 0.0064 | -2.9026 | 0.0197 | -4.3976 | 0.0029 | -0.5066 | 0.0030 | -0.1735 | 0.0111 | 0.7476 | 0.0135 | 0.7889 | 0.0075 | -0.2825 | 0.0083 | -0.6528 | 0.0344 | -0.4990 | 0.3378 | -0.1921 | 0.0433 | -0.5076 | 0.0032 | -0.0302 | 0.0134 | -0.3580 | 0.0073 | -0.0780 | 0.0089 | -1.2930 | 0.0046 | -0.1056 | 0.0695 | 0.1698 | 0.0156 | 0.6075 | 0.0126 | 0.8786 | 0.1555 | 0.4159 | 0.0134 | -0.3293 |
PLX-4720 | COLO 858 | 1.0000 | uM | 5 | hr | 1 | InnoScan/Mapix | 0.0131 | -2.0545 | 0.1341 | -2.0544 | 0.2221 | 1.0030 | 0.0316 | 1.6309 | 0.0327 | 2.0317 | 0.6538 | 0.6284 | 0.0404 | -0.4865 | 0.2410 | -0.6169 | 0.0597 | -0.1243 | 0.0084 | 0.9068 | 0.0185 | 0.1932 | 0.0110 | 0.4809 | 0.0260 | 0.4019 | 0.0056 | 0.0395 | 0.0753 | 0.2987 | 0.0444 | 1.7898 | 1.2105 | 0.1248 | 0.7443 | 0.9288 | ||||||
PLX-4720 | COLO 858 | 1.0000 | uM | 5 | hr | 2 | InnoScan/Mapix | 0.0123 | -2.0545 | -2.0544 | 0.0049 | 0.2221 | 0.0069 | 1.0030 | 0.0311 | 1.6309 | 0.0380 | 2.0317 | 0.0159 | 0.6538 | 0.0236 | 0.6284 | 0.0595 | -0.4865 | 0.3040 | -0.6169 | 0.0576 | -0.1243 | 0.0062 | 0.9068 | 0.0199 | 0.1932 | 0.0099 | 0.4809 | 0.0315 | 0.4019 | 0.0062 | 0.0395 | 0.1029 | 0.2987 | 0.0326 | 1.7898 | 0.0164 | 1.2105 | 0.2169 | 0.7443 | 0.0338 | 0.9288 | |
PLX-4720 | COLO 858 | 1.0000 | uM | 5 | hr | 3 | InnoScan/Mapix | 0.0114 | -2.0545 | 0.1309 | -2.0544 | 0.0050 | 0.2221 | 0.0077 | 1.0030 | 0.0205 | 1.6309 | 0.0304 | 2.0317 | 0.0129 | 0.6538 | 0.0233 | 0.6284 | 0.0428 | -0.4865 | 0.2551 | -0.6169 | 0.0542 | -0.1243 | 0.0045 | 0.9068 | 0.0196 | 0.1932 | 0.0108 | 0.4809 | 0.0288 | 0.4019 | 0.0100 | 0.0395 | 0.0676 | 0.2987 | 0.0340 | 1.7898 | 0.0151 | 1.2105 | 0.1230 | 0.7443 | 0.0319 | 0.9288 |
PLX-4720 | COLO 858 | 1.0000 | uM | 5 | hr | 4 | InnoScan/Mapix | 0.0102 | -2.0545 | 0.1335 | -2.0544 | 0.0042 | 0.2221 | 0.0049 | 1.0030 | 0.0222 | 1.6309 | 2.0317 | 0.0141 | 0.6538 | 0.0235 | 0.6284 | 0.0625 | -0.4865 | 0.2315 | -0.6169 | 0.0577 | -0.1243 | 0.0057 | 0.9068 | 0.0191 | 0.1932 | 0.0100 | 0.4809 | 0.0342 | 0.4019 | 0.0059 | 0.0395 | 0.0715 | 0.2987 | 0.0364 | 1.7898 | 0.0142 | 1.2105 | 0.1590 | 0.7443 | 0.0326 | 0.9288 | |
PLX-4720 | COLO 858 | 1.0000 | uM | 5 | hr | 5 | InnoScan/Mapix | 0.0116 | -2.0545 | 0.0830 | -2.0544 | 0.0043 | 0.2221 | 0.0075 | 1.0030 | 0.0192 | 1.6309 | 0.0261 | 2.0317 | 0.0134 | 0.6538 | 0.0241 | 0.6284 | 0.0438 | -0.4865 | 0.1965 | -0.6169 | 0.0559 | -0.1243 | 0.0059 | 0.9068 | 0.0168 | 0.1932 | 0.0109 | 0.4809 | 0.0237 | 0.4019 | 0.0055 | 0.0395 | 0.0547 | 0.2987 | 0.0319 | 1.7898 | 0.0156 | 1.2105 | 0.0941 | 0.7443 | 0.0305 | 0.9288 |
PLX-4720 | COLO 858 | 1.0000 | uM | 5 | hr | 6 | InnoScan/Mapix | -2.0545 | 0.1014 | -2.0544 | 0.0032 | 0.2221 | 0.0044 | 1.0030 | 0.0188 | 1.6309 | 0.0315 | 2.0317 | 0.0131 | 0.6538 | 0.6284 | 0.0783 | -0.4865 | 0.1961 | -0.6169 | 0.0614 | -0.1243 | 0.0042 | 0.9068 | 0.0178 | 0.1932 | 0.4809 | 0.0277 | 0.4019 | 0.0065 | 0.0395 | 0.0710 | 0.2987 | 0.0302 | 1.7898 | 0.0120 | 1.2105 | 0.1454 | 0.7443 | 0.9288 | ||||
PLX-4720 | COLO 858 | 1.0000 | uM | 5 | hr | 7 | InnoScan/Mapix | 0.0122 | -2.0545 | 0.1029 | -2.0544 | 0.0049 | 0.2221 | 0.0068 | 1.0030 | 0.0217 | 1.6309 | 0.0319 | 2.0317 | 0.0144 | 0.6538 | 0.0243 | 0.6284 | 0.0420 | -0.4865 | 0.3226 | -0.6169 | 0.0524 | -0.1243 | 0.0059 | 0.9068 | 0.0219 | 0.1932 | 0.4809 | 0.0290 | 0.4019 | 0.0084 | 0.0395 | 0.0861 | 0.2987 | 0.0410 | 1.7898 | 0.0181 | 1.2105 | 0.1894 | 0.7443 | 0.0310 | 0.9288 | |
PLX-4720 | COLO 858 | 1.0000 | uM | 5 | hr | 8 | InnoScan/Mapix | 0.0119 | -2.0545 | 0.1089 | -2.0544 | 0.0049 | 0.2221 | 0.0073 | 1.0030 | 0.0249 | 1.6309 | 0.0369 | 2.0317 | 0.0167 | 0.6538 | 0.0239 | 0.6284 | 0.0477 | -0.4865 | 0.3165 | -0.6169 | 0.0560 | -0.1243 | 0.0068 | 0.9068 | 0.0222 | 0.1932 | 0.0110 | 0.4809 | 0.0305 | 0.4019 | 0.0066 | 0.0395 | 0.0837 | 0.2987 | 0.0473 | 1.7898 | 0.0171 | 1.2105 | 0.2370 | 0.7443 | 0.0330 | 0.9288 |
PLX-4720 | COLO 858 | 1.0000 | uM | 10 | hr | 1 | InnoScan/Mapix | 0.0084 | -2.5166 | 0.0172 | -4.4294 | 0.0054 | -0.2877 | 0.0072 | 0.4639 | 0.0137 | 0.9529 | 0.0107 | 0.7446 | 0.0101 | 0.1752 | 0.0192 | -0.0081 | 0.0308 | -0.9153 | 0.0602 | -2.9145 | 0.0873 | 0.2217 | 0.0045 | 0.2197 | 0.0091 | -0.7204 | 0.0069 | -0.0863 | 0.0105 | -0.7841 | 0.0054 | -0.0772 | 0.0725 | 0.3558 | 0.0325 | 1.7043 | 0.6879 | 0.0723 | 0.1179 | 0.0387 | 0.8518 | |
PLX-4720 | COLO 858 | 1.0000 | uM | 10 | hr | 2 | InnoScan/Mapix | 0.0086 | -2.5166 | 0.0203 | -4.4294 | 0.0030 | -0.2877 | 0.0033 | 0.4639 | 0.0137 | 0.9529 | 0.0130 | 0.7446 | 0.0134 | 0.1752 | 0.0131 | -0.0081 | 0.0333 | -0.9153 | -2.9145 | 0.0688 | 0.2217 | 0.0034 | 0.2197 | 0.0105 | -0.7204 | 0.0066 | -0.0863 | 0.0126 | -0.7841 | 0.0049 | -0.0772 | 0.1072 | 0.3558 | 0.0282 | 1.7043 | 0.0122 | 0.6879 | 0.1309 | 0.1179 | 0.0323 | 0.8518 | |
PLX-4720 | COLO 858 | 1.0000 | uM | 10 | hr | 3 | InnoScan/Mapix | 0.0087 | -2.5166 | 0.0186 | -4.4294 | 0.0032 | -0.2877 | 0.0039 | 0.4639 | 0.0119 | 0.9529 | 0.0097 | 0.7446 | 0.0080 | 0.1752 | 0.0129 | -0.0081 | 0.0354 | -0.9153 | 0.0504 | -2.9145 | 0.0718 | 0.2217 | 0.0032 | 0.2197 | 0.0100 | -0.7204 | 0.0105 | -0.0863 | 0.0113 | -0.7841 | 0.0071 | -0.0772 | 0.0685 | 0.3558 | 0.0290 | 1.7043 | 0.0087 | 0.6879 | 0.0729 | 0.1179 | 0.0249 | 0.8518 |
PLX-4720 | COLO 858 | 1.0000 | uM | 10 | hr | 4 | InnoScan/Mapix | 0.0082 | -2.5166 | 0.0145 | -4.4294 | 0.0027 | -0.2877 | 0.0032 | 0.4639 | 0.0126 | 0.9529 | 0.0132 | 0.7446 | 0.0091 | 0.1752 | 0.0115 | -0.0081 | -0.9153 | 0.0478 | -2.9145 | 0.2217 | 0.0031 | 0.2197 | 0.0100 | -0.7204 | 0.0073 | -0.0863 | 0.0139 | -0.7841 | 0.0052 | -0.0772 | 0.0748 | 0.3558 | 0.0301 | 1.7043 | 0.0092 | 0.6879 | 0.0943 | 0.1179 | 0.0269 | 0.8518 |