Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Gefitinib | MCF 10A | 10.0 | uM | 24 | hr | 192.99 | 28.34 | p-Akt | 22.21 | |
| Triciribine | T47D | 1.0 | uM | 6 | hr | 101.59 | 28.35 | p-4EBP1 | 83.39 | |
| Dactolisib | T47D | 0 (control) | 6 | hr | 88.35 | 28.53 | p-4EBP1 | 84.26 | | |
| MK2206 | T47D | 10.0 | uM | 6 | hr | 110.32 | 28.55 | p-Akt | 6.57 | |
| Torkinib | HCC1954 | 31.6 | nM | 6 | hr | 162.33 | 28.59 | p-Akt | 14.31 | |
| Dactolisib | T47D | 3.16 | nM | 6 | hr | 88.78 | 28.61 | p-S6 | 40.04 | |
| Gefitinib | HCC1954 | 3.16 | nM | 6 | hr | 131.67 | 28.62 | p-4EBP1 | 36.83 | |
| Torkinib | HCC1954 | 31.6 | nM | 6 | hr | 150.07 | 28.76 | p-Akt | 14.38 | |
| Torkinib | HCC1954 | 31.6 | nM | 6 | hr | 150.95 | 28.84 | p-S6 | 27.08 | |
| Torkinib | HCC1954 | 1.78 | nM | 6 | hr | 160.33 | 28.86 | p-Akt | 17.59 | |
| Torkinib | HCC1954 | 7.5 | nM | 6 | hr | 166.69 | 28.86 | p-Akt | 16.56 | |
| MK2206 | T47D | 3.16 | uM | 6 | hr | 102.31 | 29.14 | p-Akt | 5.87 | |
| Dactolisib | T47D | 0 (control) | 6 | hr | 89.64 | 29.16 | p-S6 | 44.67 | | |
| Dactolisib | T47D | 31.6 | nM | 6 | hr | 90.16 | 29.57 | p-S6 | 4.68 | |
| MK2206 | T47D | 10.0 | uM | 6 | hr | 105.81 | 29.63 | p-4EBP1 | 65.78 | |
| Gefitinib | MCF 10A | 1.0 | uM | 24 | hr | 158.54 | 29.78 | p-Akt | 17.71 | |
| MK2206 | T47D | 1.0 | uM | 6 | hr | 93.95 | 29.78 | p-4EBP1 | 59.88 | |
| MK2206 | HCC1954 | 0 (control) | 6 | hr | 142.39 | 29.98 | p-4EBP1 | 49.89 | | |
| Dactolisib | T47D | 100.0 | nM | 6 | hr | 80.72 | 30.01 | p-S6 | 3.74 | |
| MK2206 | T47D | 10.0 | uM | 6 | hr | 108.84 | 30.13 | p-4EBP1 | 73.21 | |
| Triciribine | T47D | 10.0 | uM | 6 | hr | 102.80 | 30.16 | p-S6 | 50.10 | |
| Gefitinib | HCC1954 | 3.16 | uM | 6 | hr | 128.34 | 30.50 | p-Akt | 19.47 | |
| Triciribine | T47D | 0 (control) | 6 | hr | 98.11 | 30.56 | p-Akt | 14.84 | | |
| Dactolisib | T47D | 1.0 | uM | 24 | hr | 115.16 | 30.63 | p-Akt | 12.53 | |
| Torkinib | HCC1954 | 31.6 | nM | 6 | hr | 161.40 | 30.73 | p-S6 | 30.08 | |