Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Dactolisib | HCC1954 | 100.0 | nM | 6 | hr | 201.86 | 59.83 | p-4EBP1 | 13.31 | |
| Dactolisib | HCC1954 | 100.0 | nM | 24 | hr | 270.29 | 86.15 | p-4EBP1 | 19.38 | |
| Gefitinib | MCF 10A | 100.0 | nM | 24 | hr | 253.20 | 163.24 | p-4EBP1 | 174.55 | |
| Torkinib | MCF 10A | 31.6 | nM | 6 | hr | 486.85 | 159.54 | p-4EBP1 | 207.18 | |
| Gefitinib | AU565 | 0 (control) | 6 | hr | 226.23 | 50.86 | p-4EBP1 | 51.79 | | |
| Gefitinib | AU565 | 0 (control) | 24 | hr | 597.19 | 141.39 | p-4EBP1 | 180.42 | | |
| Torkinib | AU565 | 0 (control) | 24 | hr | 520.08 | 134.68 | p-4EBP1 | 226.94 | | |
| GSK1059615 | AU565 | 0 (control) | 6 | hr | 234.34 | 56.31 | p-4EBP1 | 61.94 | | |
| MK2206 | AU565 | 0 (control) | 24 | hr | 443.02 | 135.36 | p-4EBP1 | 212.85 | | |
| Triciribine | AU565 | 0 (control) | 6 | hr | 277.93 | 72.56 | p-4EBP1 | 95.48 | | |
| Dactolisib | AU565 | 0 (control) | 6 | hr | 223.15 | 50.37 | p-4EBP1 | 55.92 | | |
| Dactolisib | AU565 | 0 (control) | 24 | hr | 527.81 | 136.32 | p-4EBP1 | 202.58 | | |
| Torkinib | HCC1954 | 0 (control) | 24 | hr | 299.58 | 124.61 | p-4EBP1 | 139.95 | | |
| GSK1059615 | HCC1954 | 0 (control) | 6 | hr | 337.11 | 90.46 | p-4EBP1 | 107.99 | | |
| MK2206 | HCC1954 | 0 (control) | 6 | hr | 142.39 | 29.98 | p-4EBP1 | 49.89 | | |
| MK2206 | HCC1954 | 0 (control) | 24 | hr | 298.17 | 123.52 | p-4EBP1 | 156.31 | | |
| Dactolisib | HCC1954 | 0 (control) | 6 | hr | 182.86 | 43.74 | p-4EBP1 | 53.60 | | |
| Dactolisib | HCC1954 | 0 (control) | 24 | hr | 323.44 | 115.77 | p-4EBP1 | 138.95 | | |
| Gefitinib | MCF 10A | 0 (control) | 24 | hr | 372.82 | 142.63 | p-4EBP1 | 369.21 | | |
| Torkinib | MCF 10A | 0 (control) | 6 | hr | 502.64 | 155.63 | p-4EBP1 | 400.80 | | |
| MK2206 | MCF 10A | 0 (control) | 24 | hr | 340.91 | 138.48 | p-4EBP1 | 378.35 | | |
| Triciribine | MCF 10A | 0 (control) | 6 | hr | 451.41 | 160.21 | p-4EBP1 | 412.08 | | |
| Dactolisib | MCF 10A | 0 (control) | 6 | hr | 469.24 | 161.27 | p-4EBP1 | 396.95 | | |
| Dactolisib | MCF 10A | 100.0 | nM | 6 | hr | 352.97 | 128.10 | p-4EBP1 | 59.80 | |
| Triciribine | MCF 10A | 100.0 | nM | 24 | hr | 233.97 | 155.96 | p-4EBP1 | 184.14 | |