Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
Download: Excel (XLSX), Comma-separated (CSV)
| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| GSK1059615 | AU565 | 3.16 | nM | 6 | hr | 218.51 | 54.15 | p-Akt | 24.23 | |
| Dactolisib | AU565 | 31.6 | nM | 6 | hr | 227.70 | 45.24 | p-Akt | 16.61 | |
| Torkinib | AU565 | 0.1 | nM | 6 | hr | 230.65 | 52.24 | p-Akt | 23.88 | |
| Gefitinib | HCC1954 | 31.6 | nM | 6 | hr | 154.47 | 31.92 | p-Akt | 21.41 | |
| MK2206 | AU565 | 3.16 | nM | 6 | hr | 255.60 | 70.38 | p-Akt | 22.59 | |
| Torkinib | HCC1954 | 7.5 | nM | 6 | hr | 166.69 | 28.86 | p-Akt | 16.56 | |
| Gefitinib | HCC1954 | 0 (control) | 6 | hr | 411.80 | 93.54 | p-Akt | 46.61 | | |
| GSK1059615 | HCC1954 | 31.6 | nM | 6 | hr | 360.63 | 99.54 | p-Akt | 54.35 | |
| Triciribine | AU565 | 3.16 | nM | 6 | hr | 205.62 | 54.24 | p-Akt | 19.22 | |
| MK2206 | HCC1954 | 31.6 | nM | 6 | hr | 155.55 | 39.66 | p-Akt | 15.51 | |
| GSK1059615 | AU565 | 1.0 | nM | 6 | hr | 277.32 | 67.90 | p-Akt | 29.88 | |
| Triciribine | HCC1954 | 31.6 | nM | 6 | hr | 227.75 | 83.99 | p-Akt | 41.27 | |
| Dactolisib | AU565 | 3.16 | nM | 6 | hr | 259.31 | 60.60 | p-Akt | 24.45 | |
| Dactolisib | HCC1954 | 31.6 | nM | 6 | hr | 236.72 | 75.54 | p-Akt | 26.13 | |
| Torkinib | MCF 10A | 0 (control) | 6 | hr | 498.87 | 147.67 | p-Akt | 54.88 | | |
| Gefitinib | MCF 10A | 31.6 | nM | 6 | hr | 398.27 | 164.91 | p-Akt | 40.24 | |
| Gefitinib | HCC1954 | 3.16 | nM | 6 | hr | 285.82 | 61.71 | p-Akt | 30.33 | |
| Torkinib | MCF 10A | 7.5 | nM | 6 | hr | 427.52 | 157.79 | p-Akt | 37.03 | |
| MK2206 | AU565 | 1.0 | nM | 6 | hr | 225.66 | 66.86 | p-Akt | 22.58 | |
| GSK1059615 | MCF 10A | 31.6 | nM | 6 | hr | 373.13 | 166.27 | p-Akt | 34.46 | |
| Torkinib | HCC1954 | 0.422 | nM | 6 | hr | 213.03 | 46.19 | p-Akt | 24.57 | |
| MK2206 | MCF 10A | 31.6 | nM | 6 | hr | 380.25 | 159.60 | p-Akt | 30.86 | |
| MK2206 | AU565 | 0 (control) | 6 | hr | 215.16 | 63.34 | p-Akt | 22.81 | | |
| Triciribine | MCF 10A | 31.6 | nM | 6 | hr | 381.96 | 160.49 | p-Akt | 30.98 | |
| GSK1059615 | HCC1954 | 10.0 | uM | 6 | hr | 362.09 | 70.59 | p-Akt | 16.31 | |