Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Dactolisib | T47D | 0 (control) | 6 | hr | 99.23 | 26.75 | p-Akt | 14.05 | | |
| Triciribine | MCF 10A | 100.0 | nM | 6 | hr | 390.18 | 153.46 | p-4EBP1 | 360.59 | |
| GSK1059615 | T47D | 1.0 | uM | 6 | hr | 158.41 | 63.89 | p-Akt | 21.30 | |
| Dactolisib | MCF 10A | 100.0 | nM | 6 | hr | 352.97 | 128.10 | p-4EBP1 | 59.80 | |
| Triciribine | T47D | 10.0 | nM | 6 | hr | 106.75 | 60.43 | p-Akt | 20.60 | |
| Gefitinib | T47D | 100.0 | nM | 6 | hr | 160.51 | 99.08 | p-4EBP1 | 483.73 | |
| MK2206 | T47D | 1.0 | uM | 6 | hr | 107.58 | 49.92 | p-Akt | 7.19 | |
| Torkinib | T47D | 31.6 | nM | 6 | hr | 152.82 | 76.33 | p-4EBP1 | 310.16 | |
| Dactolisib | T47D | 1.0 | nM | 6 | hr | 90.24 | 35.16 | p-Akt | 13.70 | |
| GSK1059615 | T47D | 100.0 | nM | 6 | hr | 161.08 | 79.36 | p-4EBP1 | 506.97 | |
| Triciribine | T47D | 1.0 | uM | 6 | hr | 119.47 | 61.20 | p-Akt | 13.24 | |
| MK2206 | T47D | 100.0 | nM | 6 | hr | 93.04 | 34.39 | p-4EBP1 | 75.18 | |
| Dactolisib | T47D | 10.0 | nM | 6 | hr | 101.93 | 57.67 | p-Akt | 14.24 | |
| Triciribine | T47D | 100.0 | nM | 6 | hr | 104.28 | 51.90 | p-4EBP1 | 172.03 | |
| Dactolisib | T47D | 1.0 | uM | 6 | hr | 90.30 | 37.28 | p-Akt | 6.14 | |
| Dactolisib | T47D | 100.0 | nM | 6 | hr | 93.80 | 43.48 | p-4EBP1 | 61.08 | |
| Torkinib | AU565 | 0 (control) | 6 | hr | 228.66 | 55.93 | p-Akt | 26.38 | | |
| Gefitinib | AU565 | 316.0 | nM | 6 | hr | 214.01 | 52.78 | p-4EBP1 | 62.72 | |
| Gefitinib | AU565 | 3.16 | uM | 6 | hr | 207.63 | 45.48 | p-Akt | 16.31 | |
| Torkinib | AU565 | 133.0 | nM | 6 | hr | 273.77 | 50.73 | p-4EBP1 | 12.35 | |
| Gefitinib | AU565 | 31.6 | nM | 6 | hr | 224.67 | 51.67 | p-Akt | 24.54 | |
| GSK1059615 | AU565 | 316.0 | nM | 6 | hr | 289.06 | 63.60 | p-4EBP1 | 86.03 | |
| Torkinib | AU565 | 2.37 | uM | 6 | hr | 226.24 | 41.45 | p-Akt | 12.62 | |
| MK2206 | AU565 | 316.0 | nM | 6 | hr | 333.41 | 71.95 | p-4EBP1 | 113.09 | |
| Gefitinib | HCC1954 | 3.16 | nM | 6 | hr | 192.10 | 43.07 | p-Akt | 19.92 | |