Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Dactolisib | T47D | 0 (control) | 6 | hr | 102.03 | 45.53 | p-4EBP1 | 121.29 | | |
| Triciribine | MCF 10A | 0 (control) | 6 | hr | 477.54 | 163.37 | p-Akt | 60.15 | | |
| Gefitinib | AU565 | 1.0 | nM | 6 | hr | 289.01 | 63.74 | p-4EBP1 | 68.42 | |
| Gefitinib | MCF 10A | 100.0 | nM | 6 | hr | 345.12 | 168.30 | p-Akt | 37.38 | |
| Torkinib | AU565 | 0.1 | nM | 6 | hr | 346.86 | 73.14 | p-4EBP1 | 98.91 | |
| Gefitinib | T47D | 3.16 | nM | 6 | hr | 181.54 | 100.89 | p-Akt | 103.21 | |
| GSK1059615 | AU565 | 1.0 | nM | 6 | hr | 261.27 | 65.61 | p-4EBP1 | 75.05 | |
| Torkinib | MCF 10A | 31.6 | nM | 6 | hr | 436.07 | 147.11 | p-Akt | 29.06 | |
| MK2206 | AU565 | 1.0 | nM | 6 | hr | 273.47 | 71.35 | p-4EBP1 | 96.49 | |
| MK2206 | HCC1954 | 1.0 | nM | 6 | hr | 158.33 | 45.19 | p-Akt | 18.61 | |
| Triciribine | AU565 | 1.0 | nM | 6 | hr | 291.94 | 77.13 | p-4EBP1 | 98.69 | |
| GSK1059615 | MCF 10A | 100.0 | nM | 6 | hr | 316.39 | 158.33 | p-Akt | 27.50 | |
| Dactolisib | AU565 | 1.0 | nM | 6 | hr | 202.47 | 47.00 | p-4EBP1 | 51.99 | |
| Torkinib | T47D | 0.422 | nM | 6 | hr | 170.81 | 90.25 | p-Akt | 78.90 | |
| Gefitinib | HCC1954 | 1.0 | nM | 6 | hr | 192.39 | 39.79 | p-4EBP1 | 52.60 | |
| MK2206 | MCF 10A | 100.0 | nM | 6 | hr | 358.46 | 151.32 | p-Akt | 25.55 | |
| Torkinib | HCC1954 | 0.1 | nM | 6 | hr | 181.17 | 40.64 | p-4EBP1 | 53.94 | |
| GSK1059615 | HCC1954 | 0 (control) | 6 | hr | 241.24 | 67.17 | p-Akt | 30.76 | | |
| GSK1059615 | HCC1954 | 1.0 | nM | 6 | hr | 373.59 | 94.92 | p-4EBP1 | 112.38 | |
| Triciribine | MCF 10A | 100.0 | nM | 6 | hr | 360.87 | 152.35 | p-Akt | 25.66 | |
| MK2206 | HCC1954 | 1.0 | nM | 6 | hr | 194.30 | 65.17 | p-4EBP1 | 85.41 | |
| GSK1059615 | T47D | 3.16 | nM | 6 | hr | 165.75 | 87.48 | p-Akt | 92.56 | |
| Triciribine | HCC1954 | 1.0 | nM | 6 | hr | 235.54 | 85.78 | p-4EBP1 | 104.82 | |
| Dactolisib | MCF 10A | 100.0 | nM | 6 | hr | 342.01 | 143.79 | p-Akt | 24.33 | |
| MK2206 | T47D | 3.16 | nM | 6 | hr | 100.59 | 50.08 | p-S6 | 61.09 | |