Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Dactolisib | AU565 | 100.0 | nM | 6 | hr | 290.65 | 59.15 | p-Akt | 17.08 | |
| Dactolisib | HCC1954 | 0 (control) | 6 | hr | 182.86 | 43.74 | p-4EBP1 | 53.60 | | |
| Torkinib | HCC1954 | 0.1 | nM | 6 | hr | 195.86 | 37.51 | p-Akt | 23.03 | |
| Gefitinib | MCF 10A | 0 (control) | 6 | hr | 434.95 | 158.69 | p-4EBP1 | 306.67 | | |
| Gefitinib | HCC1954 | 100.0 | nM | 6 | hr | 166.09 | 41.51 | p-Akt | 23.50 | |
| Torkinib | MCF 10A | 0 (control) | 6 | hr | 502.64 | 155.63 | p-4EBP1 | 400.80 | | |
| MK2206 | MCF 10A | 3.16 | nM | 6 | hr | 401.94 | 154.74 | p-Akt | 43.87 | |
| GSK1059615 | MCF 10A | 0 (control) | 6 | hr | 454.39 | 167.02 | p-4EBP1 | 405.75 | | |
| Torkinib | HCC1954 | 31.6 | nM | 6 | hr | 150.07 | 28.76 | p-Akt | 14.38 | |
| MK2206 | MCF 10A | 0 (control) | 6 | hr | 448.81 | 160.03 | p-4EBP1 | 410.77 | | |
| Gefitinib | AU565 | 0 (control) | 6 | hr | 286.03 | 62.73 | p-Akt | 29.07 | | |
| Triciribine | MCF 10A | 0 (control) | 6 | hr | 451.41 | 160.21 | p-4EBP1 | 412.08 | | |
| GSK1059615 | HCC1954 | 100.0 | nM | 6 | hr | 324.83 | 87.89 | p-Akt | 34.01 | |
| Dactolisib | MCF 10A | 0 (control) | 6 | hr | 469.24 | 161.27 | p-4EBP1 | 396.95 | | |
| Triciribine | MCF 10A | 3.16 | nM | 6 | hr | 404.45 | 155.90 | p-Akt | 44.15 | |
| Gefitinib | T47D | 0 (control) | 6 | hr | 187.68 | 101.38 | p-4EBP1 | 462.97 | | |
| MK2206 | HCC1954 | 100.0 | nM | 6 | hr | 239.59 | 77.83 | p-Akt | 17.44 | |
| Torkinib | T47D | 0 (control) | 6 | hr | 178.21 | 89.61 | p-4EBP1 | 518.71 | | |
| GSK1059615 | HCC1954 | 1.0 | nM | 6 | hr | 251.93 | 70.14 | p-Akt | 31.99 | |
| GSK1059615 | T47D | 0 (control) | 6 | hr | 170.99 | 85.49 | p-4EBP1 | 487.39 | | |
| Triciribine | HCC1954 | 100.0 | nM | 6 | hr | 200.10 | 73.80 | p-Akt | 29.38 | |
| MK2206 | T47D | 0 (control) | 6 | hr | 108.26 | 31.89 | p-4EBP1 | 66.68 | | |
| Dactolisib | MCF 10A | 3.16 | nM | 6 | hr | 394.32 | 173.00 | p-Akt | 43.59 | |
| Triciribine | T47D | 0 (control) | 6 | hr | 91.82 | 35.81 | p-4EBP1 | 108.61 | | |
| GSK1059615 | T47D | 1.0 | nM | 6 | hr | 169.47 | 94.04 | p-S6 | 455.83 | |