Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| MK2206 | AU565 | 10.0 | nM | 6 | hr | 183.97 | 46.00 | p-Akt | 14.47 | |
| Torkinib | HCC1954 | 133.0 | nM | 6 | hr | 174.96 | 31.04 | p-Akt | 14.51 | |
| MK2206 | HCC1954 | 0 (control) | 6 | hr | 271.78 | 84.99 | p-Akt | 42.06 | | |
| GSK1059615 | HCC1954 | 316.0 | nM | 6 | hr | 348.89 | 81.09 | p-Akt | 24.10 | |
| Triciribine | AU565 | 10.0 | nM | 6 | hr | 278.40 | 74.05 | p-Akt | 23.07 | |
| MK2206 | HCC1954 | 316.0 | nM | 6 | hr | 223.77 | 64.61 | p-Akt | 15.70 | |
| GSK1059615 | MCF 10A | 1.0 | nM | 6 | hr | 470.40 | 164.01 | p-Akt | 53.70 | |
| Triciribine | HCC1954 | 316.0 | nM | 6 | hr | 241.79 | 82.96 | p-Akt | 31.94 | |
| Dactolisib | AU565 | 10.0 | nM | 6 | hr | 210.20 | 46.30 | p-Akt | 19.17 | |
| Dactolisib | HCC1954 | 316.0 | nM | 6 | hr | 172.52 | 45.49 | p-Akt | 15.11 | |
| Torkinib | T47D | 0 (control) | 6 | hr | 192.56 | 91.57 | p-Akt | 104.03 | | |
| Gefitinib | MCF 10A | 316.0 | nM | 6 | hr | 215.43 | 158.10 | p-Akt | 29.68 | |
| Gefitinib | HCC1954 | 10.0 | nM | 6 | hr | 169.47 | 33.97 | p-Akt | 21.97 | |
| Torkinib | MCF 10A | 133.0 | nM | 6 | hr | 466.79 | 144.13 | p-Akt | 22.20 | |
| MK2206 | MCF 10A | 1.0 | nM | 6 | hr | 436.98 | 158.45 | p-Akt | 51.09 | |
| GSK1059615 | MCF 10A | 316.0 | nM | 6 | hr | 259.69 | 149.07 | p-Akt | 21.21 | |
| Torkinib | HCC1954 | 1.78 | nM | 6 | hr | 160.33 | 28.86 | p-Akt | 17.59 | |
| MK2206 | MCF 10A | 316.0 | nM | 6 | hr | 361.83 | 149.71 | p-Akt | 20.90 | |
| GSK1059615 | AU565 | 0 (control) | 6 | hr | 264.31 | 61.82 | p-Akt | 28.75 | | |
| Triciribine | MCF 10A | 316.0 | nM | 6 | hr | 364.57 | 150.90 | p-Akt | 21.02 | |
| GSK1059615 | HCC1954 | 10.0 | nM | 6 | hr | 303.36 | 83.16 | p-Akt | 39.46 | |
| Dactolisib | MCF 10A | 316.0 | nM | 6 | hr | 305.65 | 142.81 | p-Akt | 19.28 | |
| Triciribine | MCF 10A | 1.0 | nM | 6 | hr | 439.88 | 158.37 | p-Akt | 51.51 | |
| Gefitinib | T47D | 316.0 | nM | 6 | hr | 157.11 | 101.80 | p-Akt | 102.46 | |
| Gefitinib | MCF 10A | 10.0 | nM | 6 | hr | 392.32 | 167.11 | p-4EBP1 | 286.87 | |