Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Triciribine | MCF 10A | 31.6 | nM | 24 | hr | 240.58 | 161.23 | p-4EBP1 | 197.24 | |
| MK2206 | HCC1954 | 10.0 | uM | 24 | hr | 296.67 | 68.02 | p-S6 | 72.61 | |
| GSK1059615 | HCC1954 | 3.16 | nM | 24 | hr | 274.26 | 111.65 | p-S6 | 305.54 | |
| Triciribine | HCC1954 | 10.0 | uM | 24 | hr | 217.54 | 89.83 | p-S6 | 389.81 | |
| Dactolisib | MCF 10A | 31.6 | nM | 24 | hr | 259.65 | 147.71 | p-4EBP1 | 83.58 | |
| Dactolisib | HCC1954 | 10.0 | uM | 24 | hr | 271.20 | 74.02 | p-S6 | 8.88 | |
| Triciribine | AU565 | 316.0 | nM | 24 | hr | 526.01 | 101.29 | p-S6 | 302.92 | |
| Gefitinib | MCF 10A | 10.0 | uM | 24 | hr | 187.17 | 27.83 | p-S6 | 16.12 | |
| Gefitinib | T47D | 31.6 | nM | 24 | hr | 153.72 | 115.23 | p-4EBP1 | 404.00 | |
| Torkinib | MCF 10A | 10.0 | uM | 24 | hr | 478.15 | 77.45 | p-S6 | 187.08 | |
| MK2206 | HCC1954 | 3.16 | nM | 24 | hr | 287.75 | 112.46 | p-S6 | 300.86 | |
| GSK1059615 | MCF 10A | 10.0 | uM | 24 | hr | 220.59 | 79.70 | p-S6 | 16.06 | |
| Torkinib | T47D | 7.5 | nM | 24 | hr | 134.56 | 75.54 | p-4EBP1 | 293.71 | |
| MK2206 | MCF 10A | 10.0 | uM | 24 | hr | 347.28 | 94.14 | p-S6 | 358.98 | |
| GSK1059615 | AU565 | 3.16 | uM | 24 | hr | 368.72 | 72.88 | p-S6 | 9.02 | |
| Triciribine | MCF 10A | 10.0 | uM | 24 | hr | 311.22 | 119.59 | p-S6 | 356.95 | |
| GSK1059615 | T47D | 31.6 | nM | 24 | hr | 144.33 | 101.31 | p-4EBP1 | 406.55 | |
| Dactolisib | MCF 10A | 10.0 | uM | 24 | hr | 431.99 | 70.22 | p-S6 | 271.08 | |
| Triciribine | HCC1954 | 3.16 | nM | 24 | hr | 300.37 | 110.91 | p-S6 | 306.04 | |
| Gefitinib | T47D | 10.0 | uM | 24 | hr | 140.88 | 93.13 | p-S6 | 272.88 | |
| MK2206 | T47D | 31.6 | nM | 24 | hr | 149.34 | 93.03 | p-4EBP1 | 348.95 | |
| Torkinib | T47D | 10.0 | uM | 24 | hr | 146.76 | 43.93 | p-S6 | 6.94 | |
| Dactolisib | AU565 | 316.0 | nM | 24 | hr | 436.33 | 59.46 | p-S6 | 12.66 | |
| GSK1059615 | T47D | 10.0 | uM | 24 | hr | 202.49 | 68.59 | p-S6 | 9.26 | |
| Triciribine | T47D | 31.6 | nM | 24 | hr | 165.90 | 102.35 | p-4EBP1 | 358.91 | |