Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Dactolisib | MCF 10A | 31.6 | nM | 6 | hr | 378.27 | 159.78 | p-Akt | 36.16 | |
| Triciribine | AU565 | 1.0 | nM | 6 | hr | 225.05 | 59.35 | p-Akt | 21.63 | |
| Gefitinib | T47D | 31.6 | nM | 6 | hr | 167.03 | 97.05 | p-Akt | 101.76 | |
| MK2206 | HCC1954 | 3.16 | nM | 6 | hr | 293.11 | 80.61 | p-Akt | 31.92 | |
| Torkinib | T47D | 7.5 | nM | 6 | hr | 157.46 | 86.43 | p-Akt | 48.47 | |
| GSK1059615 | MCF 10A | 0 (control) | 6 | hr | 450.57 | 164.32 | p-Akt | 55.84 | | |
| GSK1059615 | T47D | 31.6 | nM | 6 | hr | 168.17 | 85.32 | p-Akt | 73.10 | |
| Triciribine | HCC1954 | 3.16 | nM | 6 | hr | 237.48 | 78.12 | p-Akt | 40.18 | |
| MK2206 | T47D | 31.6 | nM | 6 | hr | 105.79 | 49.19 | p-Akt | 9.27 | |
| Dactolisib | AU565 | 1.0 | nM | 6 | hr | 217.79 | 50.25 | p-Akt | 22.45 | |
| Triciribine | T47D | 31.6 | nM | 6 | hr | 111.54 | 58.38 | p-Akt | 17.15 | |
| Dactolisib | HCC1954 | 3.16 | nM | 6 | hr | 218.96 | 75.61 | p-Akt | 41.58 | |
| Dactolisib | T47D | 31.6 | nM | 6 | hr | 88.70 | 45.24 | p-Akt | 10.08 | |
| Torkinib | HCC1954 | 0 (control) | 6 | hr | 182.67 | 40.88 | p-Akt | 23.74 | | |
| Gefitinib | AU565 | 100.0 | nM | 6 | hr | 221.28 | 52.62 | p-Akt | 23.51 | |
| Gefitinib | MCF 10A | 3.16 | nM | 6 | hr | 471.10 | 159.36 | p-Akt | 45.78 | |
| Torkinib | AU565 | 31.6 | nM | 6 | hr | 280.52 | 52.21 | p-Akt | 15.00 | |
| Gefitinib | HCC1954 | 1.0 | nM | 6 | hr | 322.82 | 72.03 | p-Akt | 33.80 | |
| GSK1059615 | AU565 | 100.0 | nM | 6 | hr | 223.60 | 51.25 | p-Akt | 23.72 | |
| Torkinib | MCF 10A | 0.422 | nM | 6 | hr | 423.90 | 157.64 | p-Akt | 44.17 | |
| MK2206 | AU565 | 100.0 | nM | 6 | hr | 217.60 | 53.69 | p-Akt | 13.47 | |
| MK2206 | MCF 10A | 0 (control) | 6 | hr | 475.25 | 162.73 | p-Akt | 59.87 | | |
| Triciribine | AU565 | 100.0 | nM | 6 | hr | 301.03 | 74.23 | p-Akt | 21.74 | |
| GSK1059615 | MCF 10A | 3.16 | nM | 6 | hr | 402.45 | 167.07 | p-Akt | 45.00 | |
| MK2206 | HCC1954 | 0 (control) | 6 | hr | 142.39 | 29.98 | p-4EBP1 | 49.89 | |