Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Dactolisib | T47D | 1.0 | nM | 24 | hr | 139.38 | 100.34 | p-4EBP1 | 323.20 | |
| Dactolisib | AU565 | 31.6 | nM | 6 | hr | 227.70 | 45.24 | p-Akt | 16.61 | |
| Dactolisib | AU565 | 31.6 | nM | 24 | hr | 380.97 | 70.29 | p-Akt | 48.32 | |
| Dactolisib | MCF 10A | 3.16 | nM | 24 | hr | 251.44 | 143.62 | p-S6 | 228.95 | |
| Dactolisib | HCC1954 | 3.16 | uM | 24 | hr | 269.72 | 61.63 | p-Akt | 17.51 | |
| Dactolisib | HCC1954 | 3.16 | uM | 6 | hr | 270.38 | 74.22 | p-Akt | 17.89 | |
| Dactolisib | HCC1954 | 3.16 | nM | 24 | hr | 287.27 | 107.15 | p-4EBP1 | 136.44 | |
| Dactolisib | HCC1954 | 10.0 | nM | 24 | hr | 334.72 | 100.93 | p-S6 | 32.09 | |
| Dactolisib | HCC1954 | 3.16 | nM | 6 | hr | 156.03 | 43.22 | p-4EBP1 | 57.72 | |
| Dactolisib | MCF 10A | 10.0 | nM | 24 | hr | 252.70 | 145.39 | p-S6 | 245.07 | |
| Dactolisib | MCF 10A | 3.16 | uM | 24 | hr | 363.80 | 58.41 | p-Akt | 16.17 | |
| Dactolisib | HCC1954 | 316.0 | nM | 24 | hr | 255.42 | 63.44 | p-Akt | 17.92 | |
| Dactolisib | AU565 | 3.16 | nM | 6 | hr | 259.31 | 60.60 | p-Akt | 24.45 | |
| Dactolisib | HCC1954 | 31.6 | nM | 6 | hr | 236.72 | 75.54 | p-Akt | 26.13 | |
| Dactolisib | HCC1954 | 31.6 | nM | 24 | hr | 279.67 | 87.04 | p-Akt | 38.76 | |
| Dactolisib | AU565 | 3.16 | nM | 24 | hr | 451.27 | 106.53 | p-Akt | 105.91 | |
| Dactolisib | HCC1954 | 31.6 | nM | 24 | hr | 297.37 | 88.73 | p-S6 | 20.91 | |
| Dactolisib | MCF 10A | 31.6 | nM | 6 | hr | 370.10 | 148.60 | p-S6 | 439.13 | |
| Dactolisib | MCF 10A | 3.16 | nM | 6 | hr | 383.57 | 152.45 | p-4EBP1 | 353.00 | |
| Dactolisib | T47D | 3.16 | nM | 6 | hr | 95.07 | 41.72 | p-4EBP1 | 113.42 | |
| Dactolisib | MCF 10A | 3.16 | nM | 24 | hr | 263.37 | 155.04 | p-4EBP1 | 239.83 | |
| Dactolisib | AU565 | 100.0 | nM | 6 | hr | 225.60 | 44.84 | p-S6 | 12.00 | |
| Dactolisib | MCF 10A | 316.0 | nM | 24 | hr | 331.36 | 49.75 | p-Akt | 15.70 | |
| Dactolisib | AU565 | 3.16 | uM | 6 | hr | 198.70 | 40.41 | p-S6 | 11.18 | |
| Dactolisib | T47D | 1.0 | uM | 24 | hr | 108.89 | 34.29 | p-S6 | 4.77 | |