Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Dactolisib | AU565 | 31.6 | nM | 6 | hr | 227.70 | 45.24 | p-Akt | 16.61 | |
| Dactolisib | HCC1954 | 1.0 | uM | 6 | hr | 235.17 | 64.66 | p-Akt | 16.95 | |
| Dactolisib | MCF 10A | 1.0 | uM | 6 | hr | 296.79 | 139.90 | p-Akt | 18.30 | |
| Dactolisib | MCF 10A | 1.0 | uM | 24 | hr | 358.73 | 45.31 | p-Akt | 14.31 | |
| Dactolisib | HCC1954 | 31.6 | nM | 24 | hr | 279.67 | 87.04 | p-Akt | 38.76 | |
| Dactolisib | AU565 | 3.16 | nM | 6 | hr | 259.31 | 60.60 | p-Akt | 24.45 | |
| Dactolisib | T47D | 3.16 | nM | 6 | hr | 112.31 | 65.77 | p-Akt | 19.14 | |
| Dactolisib | AU565 | 1.0 | nM | 6 | hr | 217.79 | 50.25 | p-Akt | 22.45 | |
| Dactolisib | AU565 | 1.0 | nM | 24 | hr | 494.41 | 109.15 | p-Akt | 109.06 | |
| Dactolisib | T47D | 3.16 | nM | 24 | hr | 138.41 | 87.20 | p-Akt | 40.75 | |
| Dactolisib | MCF 10A | 3.16 | uM | 6 | hr | 289.93 | 138.14 | p-Akt | 17.26 | |
| Dactolisib | AU565 | 3.16 | nM | 24 | hr | 451.27 | 106.53 | p-Akt | 105.91 | |
| Dactolisib | AU565 | 10.0 | nM | 6 | hr | 210.20 | 46.30 | p-Akt | 19.17 | |
| Dactolisib | HCC1954 | 10.0 | nM | 6 | hr | 191.42 | 72.50 | p-Akt | 29.37 | |
| Dactolisib | T47D | 1.0 | nM | 24 | hr | 138.24 | 90.29 | p-Akt | 47.16 | |
| Dactolisib | T47D | 10.0 | nM | 24 | hr | 132.09 | 76.49 | p-Akt | 31.82 | |
| Dactolisib | T47D | 31.6 | nM | 6 | hr | 88.70 | 45.24 | p-Akt | 10.08 | |
| Dactolisib | AU565 | 10.0 | nM | 24 | hr | 432.77 | 86.99 | p-Akt | 78.39 | |
| Dactolisib | MCF 10A | 0 (control) | 6 | hr | 436.72 | 165.98 | p-Akt | 55.27 | | |
| Dactolisib | HCC1954 | 1.0 | nM | 6 | hr | 151.35 | 38.59 | p-Akt | 20.04 | |
| Dactolisib | HCC1954 | 1.0 | nM | 24 | hr | 302.85 | 101.27 | p-Akt | 76.62 | |
| Dactolisib | MCF 10A | 0 (control) | 24 | hr | 351.94 | 125.18 | p-Akt | 50.36 | | |
| Dactolisib | HCC1954 | 3.16 | nM | 6 | hr | 218.96 | 75.61 | p-Akt | 41.58 | |
| Dactolisib | HCC1954 | 10.0 | uM | 6 | hr | 262.10 | 75.51 | p-Akt | 16.71 | |
| Dactolisib | AU565 | 10.0 | uM | 24 | hr | 452.82 | 70.98 | p-Akt | 21.40 | |