Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Gefitinib | MCF 10A | 1.0 | nM | 24 | hr | 305.19 | 131.65 | p-Akt | 50.78 | |
| Gefitinib | AU565 | 10.0 | nM | 24 | hr | 578.95 | 132.32 | p-4EBP1 | 159.55 | |
| Gefitinib | AU565 | 100.0 | nM | 24 | hr | 438.46 | 106.15 | p-Akt | 119.62 | |
| Gefitinib | MCF 10A | 3.16 | nM | 6 | hr | 471.10 | 159.36 | p-Akt | 45.78 | |
| Gefitinib | HCC1954 | 316.0 | nM | 24 | hr | 249.17 | 105.94 | p-4EBP1 | 124.32 | |
| Gefitinib | HCC1954 | 100.0 | nM | 6 | hr | 166.09 | 41.51 | p-Akt | 23.50 | |
| Gefitinib | AU565 | 0 (control) | 24 | hr | 597.19 | 141.39 | p-4EBP1 | 180.42 | | |
| Gefitinib | AU565 | 100.0 | nM | 6 | hr | 221.28 | 52.62 | p-Akt | 23.51 | |
| Gefitinib | MCF 10A | 1.0 | nM | 24 | hr | 355.52 | 156.59 | p-S6 | 408.54 | |
| Gefitinib | AU565 | 0 (control) | 6 | hr | 226.23 | 50.86 | p-4EBP1 | 51.79 | | |
| Gefitinib | MCF 10A | 1.0 | nM | 6 | hr | 406.40 | 163.48 | p-Akt | 45.06 | |
| Gefitinib | T47D | 1.0 | nM | 6 | hr | 186.25 | 95.12 | p-Akt | 100.55 | |
| Gefitinib | MCF 10A | 3.16 | nM | 6 | hr | 404.76 | 165.56 | p-Akt | 42.14 | |
| Gefitinib | T47D | 3.16 | nM | 24 | hr | 156.92 | 127.73 | p-4EBP1 | 422.23 | |
| Gefitinib | T47D | 3.16 | nM | 6 | hr | 186.66 | 102.68 | p-4EBP1 | 497.08 | |
| Gefitinib | AU565 | 1.0 | nM | 24 | hr | 634.91 | 132.12 | p-S6 | 494.50 | |
| Gefitinib | T47D | 100.0 | nM | 24 | hr | 143.88 | 111.89 | p-4EBP1 | 390.92 | |
| Gefitinib | AU565 | 1.0 | nM | 6 | hr | 329.62 | 68.74 | p-Akt | 34.37 | |
| Gefitinib | AU565 | 1.0 | nM | 24 | hr | 594.52 | 125.03 | p-Akt | 142.48 | |
| Gefitinib | T47D | 100.0 | nM | 6 | hr | 160.51 | 99.08 | p-4EBP1 | 483.73 | |
| Gefitinib | AU565 | 1.0 | nM | 6 | hr | 331.93 | 71.96 | p-S6 | 204.40 | |
| Gefitinib | HCC1954 | 1.0 | nM | 6 | hr | 274.52 | 71.74 | p-Akt | 25.25 | |
| Gefitinib | T47D | 10.0 | uM | 24 | hr | 145.66 | 74.25 | p-Akt | 69.13 | |
| Gefitinib | T47D | 0 (control) | 24 | hr | 170.59 | 100.50 | p-Akt | 77.77 | | |
| Gefitinib | T47D | 10.0 | uM | 6 | hr | 153.89 | 90.74 | p-Akt | 104.14 | |