Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Dactolisib | HCC1954 | 100.0 | nM | 24 | hr | 240.30 | 68.59 | p-Akt | 21.56 | |
| Dactolisib | T47D | 1.0 | nM | 24 | hr | 138.24 | 90.29 | p-Akt | 47.16 | |
| Dactolisib | HCC1954 | 1.0 | nM | 24 | hr | 302.85 | 101.27 | p-Akt | 76.62 | |
| Dactolisib | T47D | 1.0 | nM | 6 | hr | 90.24 | 35.16 | p-Akt | 13.70 | |
| Dactolisib | MCF 10A | 3.16 | nM | 6 | hr | 394.32 | 173.00 | p-Akt | 43.59 | |
| Dactolisib | AU565 | 1.0 | nM | 6 | hr | 217.79 | 50.25 | p-Akt | 22.45 | |
| Dactolisib | AU565 | 3.16 | nM | 6 | hr | 259.31 | 60.60 | p-Akt | 24.45 | |
| Dactolisib | AU565 | 10.0 | nM | 6 | hr | 210.20 | 46.30 | p-Akt | 19.17 | |
| Dactolisib | MCF 10A | 0 (control) | 6 | hr | 436.72 | 165.98 | p-Akt | 55.27 | | |
| Dactolisib | MCF 10A | 0 (control) | 24 | hr | 351.94 | 125.18 | p-Akt | 50.36 | | |
| Dactolisib | AU565 | 0 (control) | 24 | hr | 496.14 | 121.03 | p-Akt | 137.91 | | |
| Dactolisib | MCF 10A | 10.0 | nM | 6 | hr | 370.75 | 172.24 | p-Akt | 40.19 | |
| Dactolisib | AU565 | 1.0 | nM | 24 | hr | 494.41 | 109.15 | p-Akt | 109.06 | |
| Dactolisib | AU565 | 31.6 | nM | 6 | hr | 227.70 | 45.24 | p-Akt | 16.61 | |
| Dactolisib | MCF 10A | 1.0 | nM | 6 | hr | 437.12 | 174.36 | p-Akt | 52.90 | |
| Dactolisib | HCC1954 | 31.6 | nM | 6 | hr | 236.72 | 75.54 | p-Akt | 26.13 | |
| Dactolisib | AU565 | 3.16 | nM | 24 | hr | 451.27 | 106.53 | p-Akt | 105.91 | |
| Dactolisib | AU565 | 0 (control) | 6 | hr | 235.49 | 54.01 | p-Akt | 23.90 | | |
| Dactolisib | MCF 10A | 1.0 | nM | 24 | hr | 332.80 | 131.47 | p-Akt | 43.15 | |
| Dactolisib | HCC1954 | 0 (control) | 6 | hr | 262.96 | 93.99 | p-Akt | 56.53 | | |
| Dactolisib | T47D | 0 (control) | 6 | hr | 99.23 | 26.75 | p-Akt | 14.05 | | |
| Dactolisib | T47D | 0 (control) | 24 | hr | 165.27 | 86.01 | p-Akt | 60.10 | | |
| Dactolisib | HCC1954 | 0 (control) | 24 | hr | 357.28 | 117.27 | p-Akt | 79.92 | | |
| Dactolisib | AU565 | 316.0 | nM | 24 | hr | 403.53 | 59.74 | p-Akt | 21.21 | |
| Dactolisib | AU565 | 316.0 | nM | 6 | hr | 300.22 | 59.06 | p-Akt | 16.85 | |