Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Dactolisib | T47D | 0 (control) | 6 | hr | 102.03 | 45.53 | p-4EBP1 | 121.29 | | |
| Dactolisib | HCC1954 | 3.16 | uM | 6 | hr | 270.38 | 74.22 | p-Akt | 17.89 | |
| Dactolisib | HCC1954 | 1.0 | nM | 24 | hr | 301.34 | 115.11 | p-4EBP1 | 140.89 | |
| Dactolisib | AU565 | 1.0 | nM | 24 | hr | 513.37 | 123.17 | p-4EBP1 | 183.62 | |
| Dactolisib | AU565 | 100.0 | nM | 24 | hr | 396.58 | 64.02 | p-4EBP1 | 26.27 | |
| Dactolisib | MCF 10A | 3.16 | uM | 6 | hr | 289.93 | 138.14 | p-Akt | 17.26 | |
| Dactolisib | AU565 | 1.0 | nM | 6 | hr | 202.47 | 47.00 | p-4EBP1 | 51.99 | |
| Dactolisib | HCC1954 | 100.0 | nM | 6 | hr | 201.86 | 59.83 | p-4EBP1 | 13.31 | |
| Dactolisib | AU565 | 10.0 | nM | 6 | hr | 210.20 | 46.30 | p-Akt | 19.17 | |
| Dactolisib | HCC1954 | 316.0 | nM | 6 | hr | 172.52 | 45.49 | p-Akt | 15.11 | |
| Dactolisib | HCC1954 | 316.0 | nM | 24 | hr | 255.42 | 63.44 | p-Akt | 17.92 | |
| Dactolisib | AU565 | 10.0 | nM | 24 | hr | 432.77 | 86.99 | p-Akt | 78.39 | |
| Dactolisib | HCC1954 | 1.0 | nM | 6 | hr | 167.72 | 46.85 | p-4EBP1 | 64.61 | |
| Dactolisib | MCF 10A | 316.0 | nM | 24 | hr | 332.74 | 49.60 | p-S6 | 216.41 | |
| Dactolisib | AU565 | 1.0 | uM | 6 | hr | 218.19 | 44.53 | p-S6 | 12.18 | |
| Dactolisib | AU565 | 1.0 | uM | 24 | hr | 442.86 | 59.90 | p-S6 | 12.23 | |
| Dactolisib | MCF 10A | 100.0 | nM | 6 | hr | 352.97 | 128.10 | p-4EBP1 | 59.80 | |
| Dactolisib | MCF 10A | 100.0 | nM | 24 | hr | 315.69 | 93.24 | p-4EBP1 | 24.59 | |
| Dactolisib | T47D | 3.16 | uM | 6 | hr | 86.04 | 36.41 | p-Akt | 6.31 | |
| Dactolisib | MCF 10A | 1.0 | nM | 6 | hr | 412.87 | 154.76 | p-4EBP1 | 376.60 | |
| Dactolisib | T47D | 1.0 | nM | 6 | hr | 95.66 | 52.97 | p-4EBP1 | 132.08 | |
| Dactolisib | MCF 10A | 316.0 | nM | 6 | hr | 305.65 | 142.81 | p-Akt | 19.28 | |
| Dactolisib | MCF 10A | 316.0 | nM | 24 | hr | 331.36 | 49.75 | p-Akt | 15.70 | |
| Dactolisib | MCF 10A | 0 (control) | 6 | hr | 444.47 | 164.51 | p-4EBP1 | 404.34 | | |
| Dactolisib | HCC1954 | 0 (control) | 24 | hr | 304.21 | 127.11 | p-4EBP1 | 143.88 | |