Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Dactolisib | MCF 10A | 10.0 | uM | 24 | hr | 432.47 | 63.35 | p-Akt | 21.43 | |
| Dactolisib | T47D | 3.16 | nM | 24 | hr | 130.71 | 105.86 | p-4EBP1 | 330.99 | |
| Dactolisib | MCF 10A | 10.0 | uM | 6 | hr | 308.31 | 134.66 | p-Akt | 16.90 | |
| Dactolisib | MCF 10A | 0 (control) | 6 | hr | 436.72 | 165.98 | p-Akt | 55.27 | | |
| Dactolisib | T47D | 10.0 | uM | 24 | hr | 144.80 | 88.36 | p-Akt | 16.01 | |
| Dactolisib | AU565 | 10.0 | nM | 6 | hr | 265.79 | 60.68 | p-4EBP1 | 71.04 | |
| Dactolisib | MCF 10A | 1.0 | uM | 6 | hr | 296.79 | 139.90 | p-Akt | 18.30 | |
| Dactolisib | HCC1954 | 1.0 | nM | 6 | hr | 151.35 | 38.59 | p-Akt | 20.04 | |
| Dactolisib | HCC1954 | 316.0 | nM | 24 | hr | 255.42 | 63.44 | p-Akt | 17.92 | |
| Dactolisib | T47D | 3.16 | nM | 6 | hr | 112.31 | 65.77 | p-Akt | 19.14 | |
| Dactolisib | T47D | 100.0 | nM | 6 | hr | 101.93 | 53.36 | p-Akt | 8.50 | |
| Dactolisib | T47D | 100.0 | nM | 24 | hr | 121.98 | 38.12 | p-Akt | 19.84 | |
| Dactolisib | T47D | 3.16 | nM | 24 | hr | 138.41 | 87.20 | p-Akt | 40.75 | |
| Dactolisib | HCC1954 | 1.0 | nM | 24 | hr | 302.85 | 101.27 | p-Akt | 76.62 | |
| Dactolisib | AU565 | 3.16 | nM | 24 | hr | 509.48 | 113.22 | p-S6 | 302.11 | |
| Dactolisib | MCF 10A | 0 (control) | 24 | hr | 351.94 | 125.18 | p-Akt | 50.36 | | |
| Dactolisib | T47D | 1.0 | uM | 24 | hr | 115.16 | 30.63 | p-Akt | 12.53 | |
| Dactolisib | HCC1954 | 0 (control) | 6 | hr | 182.86 | 43.74 | p-4EBP1 | 53.60 | | |
| Dactolisib | MCF 10A | 3.16 | nM | 24 | hr | 251.44 | 143.62 | p-S6 | 228.95 | |
| Dactolisib | AU565 | 0 (control) | 6 | hr | 223.15 | 50.37 | p-4EBP1 | 55.92 | | |
| Dactolisib | T47D | 1.0 | uM | 6 | hr | 90.30 | 37.28 | p-Akt | 6.14 | |
| Dactolisib | MCF 10A | 10.0 | nM | 6 | hr | 347.50 | 141.22 | p-4EBP1 | 316.53 | |
| Dactolisib | AU565 | 316.0 | nM | 6 | hr | 300.22 | 59.06 | p-Akt | 16.85 | |
| Dactolisib | HCC1954 | 1.0 | uM | 24 | hr | 266.78 | 61.07 | p-Akt | 16.68 | |
| Dactolisib | HCC1954 | 1.0 | uM | 6 | hr | 209.83 | 60.36 | p-Akt | 16.60 | |