Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Triciribine | HCC1954 | 1.0 | nM | 6 | hr | 163.46 | 53.08 | p-4EBP1 | 72.71 | |
| Triciribine | MCF 10A | 31.6 | nM | 6 | hr | 381.96 | 160.49 | p-Akt | 30.98 | |
| Triciribine | HCC1954 | 10.0 | uM | 6 | hr | 212.56 | 61.42 | p-4EBP1 | 95.54 | |
| Triciribine | HCC1954 | 10.0 | uM | 24 | hr | 202.87 | 94.00 | p-4EBP1 | 110.02 | |
| Triciribine | MCF 10A | 31.6 | nM | 24 | hr | 257.99 | 128.96 | p-Akt | 24.48 | |
| Triciribine | AU565 | 1.0 | nM | 24 | hr | 496.90 | 135.91 | p-4EBP1 | 227.34 | |
| Triciribine | AU565 | 1.0 | nM | 6 | hr | 371.79 | 87.94 | p-4EBP1 | 131.82 | |
| Triciribine | T47D | 0 (control) | 24 | hr | 151.15 | 101.76 | p-4EBP1 | 381.76 | | |
| Triciribine | HCC1954 | 1.0 | nM | 6 | hr | 273.39 | 95.53 | p-S6 | 432.10 | |
| Triciribine | HCC1954 | 1.0 | nM | 24 | hr | 295.65 | 96.48 | p-Akt | 59.73 | |
| Triciribine | T47D | 0 (control) | 6 | hr | 96.15 | 34.17 | p-4EBP1 | 92.30 | | |
| Triciribine | MCF 10A | 0 (control) | 24 | hr | 306.71 | 141.21 | p-4EBP1 | 333.89 | | |
| Triciribine | MCF 10A | 0 (control) | 6 | hr | 480.71 | 167.76 | p-4EBP1 | 402.88 | | |
| Triciribine | HCC1954 | 0 (control) | 24 | hr | 320.03 | 109.53 | p-4EBP1 | 131.32 | | |
| Triciribine | MCF 10A | 10.0 | uM | 6 | hr | 355.57 | 132.02 | p-4EBP1 | 308.01 | |
| Triciribine | MCF 10A | 10.0 | uM | 24 | hr | 251.64 | 109.25 | p-4EBP1 | 200.79 | |
| Triciribine | HCC1954 | 0 (control) | 6 | hr | 263.52 | 82.77 | p-4EBP1 | 106.42 | | |
| Triciribine | T47D | 31.6 | nM | 6 | hr | 111.54 | 58.38 | p-Akt | 17.15 | |
| Triciribine | MCF 10A | 3.16 | uM | 6 | hr | 370.92 | 146.93 | p-Akt | 17.67 | |
| Triciribine | MCF 10A | 3.16 | uM | 24 | hr | 279.48 | 109.63 | p-Akt | 20.65 | |
| Triciribine | AU565 | 100.0 | nM | 24 | hr | 594.21 | 121.58 | p-S6 | 442.44 | |
| Triciribine | HCC1954 | 1.0 | uM | 6 | hr | 262.74 | 87.39 | p-4EBP1 | 128.31 | |
| Triciribine | T47D | 31.6 | nM | 24 | hr | 173.98 | 88.45 | p-Akt | 50.47 | |
| Triciribine | AU565 | 0 (control) | 24 | hr | 473.12 | 123.96 | p-4EBP1 | 227.44 | | |
| Triciribine | AU565 | 0 (control) | 6 | hr | 225.03 | 56.04 | p-4EBP1 | 66.91 | |