Breast cell line dose response to target inhibition measured by high throughput microscopy - Dataset (ID:20136)
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| Small Molecule | Cell Line | Drug Concentration | Conc Units | Timepoint | Timepoint Units | Measured pERK (Alexa 488) | Measured p-Rb (Alexa 568) | Alexa Fluor 647 | Measured p-Akt/p-4EBP1/p-S6 (Alexa 647) | Image |
|---|---|---|---|---|---|---|---|---|---|---|
| Dactolisib | AU565 | 100.0 | nM | 24 | hr | 377.63 | 60.16 | p-Akt | 25.52 | |
| Dactolisib | AU565 | 1.0 | nM | 24 | hr | 513.37 | 123.17 | p-4EBP1 | 183.62 | |
| Dactolisib | MCF 10A | 316.0 | nM | 24 | hr | 319.08 | 53.99 | p-4EBP1 | 8.38 | |
| Dactolisib | AU565 | 1.0 | nM | 6 | hr | 202.47 | 47.00 | p-4EBP1 | 51.99 | |
| Dactolisib | MCF 10A | 100.0 | nM | 6 | hr | 342.01 | 143.79 | p-Akt | 24.33 | |
| Dactolisib | AU565 | 3.16 | nM | 6 | hr | 213.94 | 51.94 | p-4EBP1 | 62.87 | |
| Dactolisib | HCC1954 | 10.0 | uM | 6 | hr | 262.10 | 75.51 | p-Akt | 16.71 | |
| Dactolisib | AU565 | 1.0 | uM | 6 | hr | 229.24 | 51.96 | p-4EBP1 | 8.38 | |
| Dactolisib | T47D | 100.0 | nM | 24 | hr | 121.98 | 38.12 | p-Akt | 19.84 | |
| Dactolisib | MCF 10A | 1.0 | uM | 6 | hr | 296.79 | 139.90 | p-Akt | 18.30 | |
| Dactolisib | MCF 10A | 3.16 | nM | 6 | hr | 394.32 | 173.00 | p-Akt | 43.59 | |
| Dactolisib | HCC1954 | 100.0 | nM | 6 | hr | 186.97 | 55.42 | p-Akt | 16.95 | |
| Dactolisib | HCC1954 | 100.0 | nM | 24 | hr | 240.30 | 68.59 | p-Akt | 21.56 | |
| Dactolisib | MCF 10A | 3.16 | nM | 24 | hr | 306.83 | 142.69 | p-Akt | 34.75 | |
| Dactolisib | MCF 10A | 1.0 | uM | 24 | hr | 358.30 | 52.88 | p-4EBP1 | 6.29 | |
| Dactolisib | T47D | 1.0 | uM | 6 | hr | 88.97 | 38.37 | p-4EBP1 | 6.28 | |
| Dactolisib | MCF 10A | 1.0 | nM | 24 | hr | 272.56 | 144.30 | p-4EBP1 | 309.29 | |
| Dactolisib | MCF 10A | 3.16 | uM | 24 | hr | 363.80 | 58.41 | p-Akt | 16.17 | |
| Dactolisib | MCF 10A | 3.16 | uM | 6 | hr | 289.93 | 138.14 | p-Akt | 17.26 | |
| Dactolisib | MCF 10A | 1.0 | nM | 6 | hr | 412.87 | 154.76 | p-4EBP1 | 376.60 | |
| Dactolisib | T47D | 316.0 | nM | 6 | hr | 92.43 | 45.00 | p-Akt | 6.96 | |
| Dactolisib | T47D | 100.0 | nM | 6 | hr | 101.93 | 53.36 | p-Akt | 8.50 | |
| Dactolisib | T47D | 3.16 | uM | 6 | hr | 86.04 | 36.41 | p-Akt | 6.31 | |
| Dactolisib | HCC1954 | 31.6 | nM | 6 | hr | 184.53 | 45.81 | p-Akt | 19.34 | |
| Dactolisib | AU565 | 31.6 | nM | 24 | hr | 459.58 | 74.34 | p-Akt | 46.97 | |